Webcast HALO

Elevate Your Separations of Basic Compounds at High pH Using New Column Chemistry

Airing 1: Thursday, May 30, 2024 at 11am EDT / 12pm CDT / 8am PDT
Airing 2: Friday, May 31, 2024 at 11am CST / 12pm JST / 1pm AEST


Event Overview

LC and LCMS methods run at elevated pH show advantages in terms of retention and peak shape symmetry for basic analytes. A new high pH stable silica phase designed on superficially porous particle technology enables separation methods using both elevated pH and temperature. Comparative stability data and application examples showing how chromatography improves when moving from low pH to high pH conditions will be presented for small molecule analytes and oligonucleotides.


Key Learning Objectives:

  • Improve the peak shape and retention of basic compounds by using a high pH stable SPP column.
  • Understand the advantages of pH as a method development lever.
  • Learn about the column options for analyzing basic analytes based upon selected pH.


Who Should Attend:

  • Scientists developing LC and LCMS methods
  • Lab managers
  • Chromatographers
  • QA/QC managers