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AflaCLEAN
The AflaCLEAN series from LCTech comprises selective immunoaffinity columns for efficient sample preparation for the analysis of aflatoxins, in which the target analytes are specifically bound to the column and separated from the matrix. Subsequent elution yields analytes that are ready for injection into an LC analysis. The AflaCLEAN SMART variants offer shorter column formats for reduced solvent consumption and faster workflows. It is also possible to connect several SMART columns in series (e.g. OtaCLEAN SMART) for the simultaneous purification of different analytes.
Technical Data
Product | Shelf life (from date of manufacture) | Storage temperature | Size | Loading capacity | Aflatoxin recoveries | LOD (limit of detection) |
|---|---|---|---|---|---|---|
AflaCLEAN | 24 months | 4 °C – 30 °C | 3 μL | 150 ng aflatoxin B1 | B1 >90%, B2 >80%, G1 >90%, G2 >80% | B1: 0.030 ng/mL, B2: 0.014 ng/mL, G1: 0.012 ng/mL, G2: 0.006 ng/mL |
AflaCLEAN SMART | 18 months | 4 °C – 8 °C | 3 cmb | 100 ng aflatoxin B1 | B1 >90%, B2 >80%, G1 >90%, G2 >80% | B1: 0.007 ng/mL, B2: 0.002 ng/mL, G1: 0.008 ng/mL, G2: 0.002 ng/mL |
AflaCLEAN Select | 12 months | 4 °C – 8 °C | 3 mLa | 200 ng aflatoxin B1 | B1 >90%, B2 >80%, G1 >90%, G2 >80% | B1: 0.031 ng/mL, B2: 0.015 ng/mL, G1: 0.014 ng/mL, G2: 0.007 ng/mL |
AflaCLEAN M1 Select | 12 months | 4 °C – 8 °C | 3 μL | 100 ng aflatoxin M1 | M1 >90% | 0.02 ng/mL |
AflaCLEAN M1 SMART | 12 months | 4 °C – 8 °C | 3 cmb | 100 ng aflatoxin M1 | M1 >80% | 0.0007 ng/mL |
Afla-OtaCLEANc | 18 months | 4 °C – 30 °C | 3 mLa | 150 ng aflatoxin B1, 200 ng ochratoxin A | B1 >90%, B2 >80%, G1 >90%, G2 >60%, Ochratoxin A >90% | B1: 0.025 ng/mL, B2: 0.014 ng/mL, G1: 0.012 ng/mL, G2: 0.006 ng/mL, OTA: 0.03 ng/mL |
a: Total volume of the IAC column, b: Total length of the IAC column, c: Mixed bed cartridge for the analysis of alfa- and ochratoxins. For ochratoxin analysis only, see OtaCLEAN.
What makes LCTech’s SMART series stand out?
LCTech’s SMART-IAC columns are based on a compact column design for immunoaffinity-based sample purification. Compared to conventional IAC columns with a 3 mL capacity, both the required sample volume of the matrix and the consumption of solvents are reduced. This allows sample preparation to be carried out with lower resource requirements. Due to the reduced column volume, the overall processing time of the method is shortened, allowing sample series to be processed more efficiently. This is particularly advantageous in analytical applications with high sample throughput. In addition, the SMART series features standardised Luer connectors, allowing multiple SMART-IAC columns to be connected in series. This enables different immunoaffinity-based selectivities to be combined in a single sample preparation step, making it possible to purify different mycotoxins in parallel within a single workflow.
The reduction in the volume of matrix and solvent used does not impair recovery rates. Following sample preparation in accordance with the LCTech protocol, the mycotoxins are obtained in a ready-to-inject form and at a concentration comparable to that of conventional 3-mL IAC purification. The sensitivity of the subsequent HPLC analysis remains unchanged, whilst the total processing time is reduced to a quarter of the original time.
Downloads
LCTech AflaCLEAN - SMART-IAC-Columns
LCTech - Mycotoxin Column Overview
Applications
LCTech AflaCLEAN - Aflatoxin Analysis with UVE
Technical Support
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LCTech Afla-OtaCLEAN - Extraction and Clean-up Procedure
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LCTech AflaCLEAN - Extraction and Clean-up Procedure
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LCTech AflaCLEAN M1 Select - Extraction and Clean-up Procedure
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LCTech AflaCLEAN M1 SMART - Extraction and Clean-up Procedure
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LCTech AflaCLEAN Select 1mL - Extraction and Clean-up Procedure
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LCTech AflaCLEAN Select 3 mL - Extraction and Clean-up Procedure
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LCTech AflaCLEAN SMART - Extraction and Clean-up Procedure
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