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Membrane Proteins
Membrane proteins are proteins that are associated with a cell membrane or membranes of cell compartments. Transmembrane proteins permeate the cell membrane completely, whereas membrane proteins are only attached to one side of the lipid bilayer.
Membrane proteins can be analysed using all separation techniques of protein analysis. These are reversed phase chromatography, hydrophilic and hydrophobic interaction chromatography (HILIC or HIC), ion exchange chromatography (IEX), size exclusion chromatography (SEC) and affinity chromatography (AFC)
Applications
Size Exclusion Chromatography of Membrane Protein Apqz in octylglucoside with Sepax columns
Peak identities
Apqz
Test conditions
Columns: Nanofilm SEC-250 300x7.8mm
SRT-C SEC-300 300x7.8mm
SRT SEC-300 300x7.8mm
Mobile phase: 20 mM TrisHCl, pH 7, 190 mM NaCl, 10 mM KCl, 40 mM octyl glucoside
Flow rate: 1 mL/min
Detection: UV, 280 nm
Injection: 2 µL with 6 mg/mL
Ion Exchange of Membrane Proteins with Tosoh TSKgel DEAE-5PW
Peak identities
Membrane proteins
Test conditions
Column: TSKgel DEAE-5PW 75x7.5mm, 10µm (07164)
Mobile phase A: 20% glycerol + octaethyleneglycol dodecylether in 20 mmol/L Tris-HCl, pH 8
Mobile phase B: 1 mol/L sodium chloride + 20% glycerol + octaethyleneglycol dodecyl ether in 20 mmol/L Tris-HCl, pH 8
Gradient: 0 to 100%B in 60 min
Flow rate: 1 mL/min
Detection: UV/Vis, 417nm
Sample: Crude extract from rat liver microsome
Downloads
Manufacturer overview
Sepax
HPLC - SEC:
- Nanofilm SEC-500, 5µm
- Nanofilm SEC-250, 5µm
- SRT-C SEC-500, 5µm
- SRT-C SEC-300, 5µm
- SRT SEC-500, 5µm
- SRT SEC-300, 5µm
Tosoh
HPLC - HIC:
- TSKgel Ether-5PW, 10µm
HPLC- IEX:
- TSKgel DEAE-5PW, 10µm
HPLC - SEC:
- TSKgel G3000SW, 10µm
For further information and application examples for analysing proteins, see Proteome analysis.
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