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Chiral chromatography and chiral HPLC columns
Chiral chromatography enables the separation of enantiomers – molecules that behave like a picture and its mirror image and cannot be superimposed. Enantiomer separation using HPLC and SFC plays a particularly important role in pharmaceutical research, drug discovery, quality control and preparative purification.
The selection of the appropriate chiral stationary phase (CSP) is crucial for successful chiral separation. Unlike in many conventional chromatographic separations, however, the suitable selectivity can only be predicted to a limited extent from the structure of the analyte. Selecting the correct chiral column is therefore often the decisive step in method development.
MZ-Analysentechnik offers one of the most comprehensive ranges of chiral HPLC and SFC columns from various manufacturers and across different selectivity classes. As an official partner of Chiral Technologies / Daicel, the world’s leading specialist in chiral chromatography, we also have direct access to a particularly broad portfolio of chiral phases and extensive application expertise.
Not sure which chiral column is right for your compound? Contact us with details of your separation requirements. We’ll assist you with column selection, application research and finding suitable alternatives.
Technical Data
Manufacturers and columns for chiral chromatography
Daicel™ - Chiral Technologies™
Daicel / Chiral Technologies is one of the world’s leading specialists in chiral chromatography and offers one of the most comprehensive ranges of chiral stationary phases. MZ-Analysentechnik is an official partner of Daicel and assists users in selecting suitable phases and column dimensions, as well as with technical queries regarding chiral method development.
The portfolio includes, amongst others, the well-known Chiralpak® and Chiralcel® columns with immobilised and coated polysaccharide phases, as well as Crownpak® and other specialised phases. This provides selectors for a very broad spectrum of chiral compounds and various chromatographic modes.
The available selector classes include polysaccharide, zwitterionic and protein-based CSPs, as well as crown ethers, anion-exchange and ligand-exchange phases.
Nouryon™ - Kromasil
Nouryon offers two particularly robust chiral stationary phases: CelluCoat™ and AmyCoat™. For CelluCoat™, the chiral selector is cellulose tris-(3,5-dimethylphenylcarbamate); for AmyCoat™, it is amylose tris-(3,5-dimethylphenylcarbamate). The stationary phases can withstand pressures of up to 400 bar and are compatible with many organic solvents without compromising the performance of the columns. Kromasil® CelluCoat™ and AmyCoat™ are available in particle sizes of 3, 5 or 10 µm, with the 10 µm material also available as bulk material.
Macherey-Nagel™
Macherey-Nagel manufactures a range of chiral stationary phases for various applications. Chiral TLC plates are also available.
| HPLC column | Chiral selector |
| Nucleodex β-OH | Undervidatised β-cyclodextrin |
| Nucleodex β-PM | Permethylated β-cyclodextrin |
| Nucleodex α-PM | Permethylated α-cyclodextrin |
| Nucleocel Delta | Cellulose tris-(3,5-dimethylphenylcarbamate) |
| Resolvosil BSA-7 | Bovine serum albumin (BSA) |
| Nucleosil CHIRAL-1 | L-hydroxyproline-Cu²⁺ complex |
| Nucleosil CHIRAL-2 | N-(3,5-dinitrobenzoyl)-D-phenylglycine |
| Nucleosil CHIRAL-3 | N-(3,5-dinitrobenzoyl)-L-phenylglycine |
Supelco™
Supelco (Merck™ formerly Sigma-Aldrich™) is a manufacturer of several special HPLC columns for enantiomer separation, which were originally developed by the company Astec in collaboration with Prof D. Armstrong from the University of Texas at Arlington. Since 2006, Astec has been part of Sigma-Aldrich, which in turn has been part of Merck KGaA since 2015.
| HPLC column | Chiral selector |
| Astec Chirobiotic® V (100 Å) Astec Chirobiotic® V2 (200 Å) | Vancomycin |
| Astec Chirobiotic® T (100 Å) Astec Chirobiotic® T2 (200 Å) | Teicoplanin |
| Astec Chirobiotic® R (100 Å) | Ristocetin |
| Astec Chirobiotic® TAG (100 Å) | Teicoplanin Aglycone |
| Astec (R,R) P-CAP™ Astec (S,S) P-CAP™ | Poly(trans-1,2-cyclohexanediyl-bis-acrylamide) |
| Astec (R,R) P-CAP™-DP Astec (S,S) P-CAP™-DP | Poly(trans-1,2-diphenyl-bis-acrylamide) |
| Astec CLC-L Astec CLC-D | Amino acid Cu(II)-complexes |
| Astec Cyclobond® I 2000 | β-Cyclodextrin |
| Astec Cyclobond® I 2000 AC | Acetylated β-cyclodextrin |
| HPLC column | Chiral selector |
| Astec Cyclobond® I 2000 DM | 2,3-O-dimethyl β-cyclodextrin |
| Astec Cyclobond® I 2000 DMP | 3,5-dimethylphenyl β-cyclodextrin |
| Astec Cyclobond® I 2000 DMP | 2,6-Dinitro-4-fluoromethylphenyl β-cyclodextrin |
| Astec Cyclobond® I 2000 HP-RSP | R,S-hydroxypropyl β-cyclodextrin |
| Astec Cyclobond® I 2000 RSP | R,S-hydroxypropyl β-cyclodextrin |
| Astec Cyclobond® I 2000 SP | S-hydroxypropyl β-cyclodextrin |
| Astec Cyclobond® II | γ-Cyclodextrin |
| Astec Cyclobond® II AC | Acetylated γ-cyclodextrin |
| Astec Cellulose DMP | Cellulosetris-(3,5-dimethylphenylcarbamate) |
Shinwa Chemical Industries Ltd
Shinwa' s best-selling chromatography column is the Ultron ES-OVM column (Art. No. 401-102), which fulfils the specifications of the United States Pharmacopoeia USP L57. Other columns for chiral chromatography are also available.
| HPLC column | Chiral selector |
| Ultron ES-OVM | Ovomucoid |
| Ultron ES-OVM-C | Ovomucoid |
| Ultron ES-Pepsin | pepsin |
| Ultron ES-BSA | Bovine serum albumin |
| Ultron ES-CD | β-Cyclodextrin |
| Ultron ES-PhCD | β-Cyclodextrin derivative |
Sumika Chemicals Analysis Service Ltd (SCAS)
Sumika™ offers special columns for chiral separations using HPLC.
| Sumichiral | Chiral selector | Sumichiral | Chiral selector |
| OA-2000 | (R)-phenylglycine | OA-2000S | (S)-phenylglycine |
| OA-2000-I | (R)-Phenylglycine | OA-2000S-I | (S)-Phenylglycine |
| OA-2500 | (R)-1-Naphtylglycine | OA-2500S | (S)-1-Naphtylglycine |
| OA-2500-I | (R)-1-Naphtylglycine | OA-2500S-I | (S)-1-Naphthylglycine |
| OA-3100 | (S)-Valine | OA-3100R | (R)-Valine |
| OA-3200 | (S)-tert-Leucine | OA-3200R | (R)-tert-Leucine |
| OA-3300 | (R)-phenylglycine | OA-3300S | (S)-phenylglycine |
| OA-4000 | (S)-Valine (S)-1-(α-naphthyl)ethylamine | OA-4000R | (R)-Valine (R)-1-(α-naphthyl)ethylamine |
| OA-4100 | (S)-Valine (R)-1-(α-naphthyl)ethylamine | OA-4100R | (R)-Valine (S)-1-(α-naphthyl)ethylamine |
| OA-4400 | (S)-Proline (S)-1-(α-naphthyl)ethylamine | OA-4400R | (R)-Proline (R)-1-(α-naphthyl)ethylamine |
| OA-4500 | (S)-Proline (R)-1-(α-naphthyl)ethylamine | OA-4500R | (R)-Proline (S)-1-(α-naphthyl)ethylamine |
| OA-4600 | (S)-tert-Leucine (S)-1-(α-naphthyl)ethylamine | OA-4600R | (R)-tert-Leucine (R)-1-(α-naphthyl)ethylamine |
| OA-4700 | (S)-tert-Leucine (R)-1-(α-naphthyl)ethylamine | OA-4700R | (R)-tert-Leucine (S)-1-(α-naphthyl)ethylamine |
| OA-4800 | (S)-Indoline-2-carboxylic acid (S)-1-(α-naphthyl)ethylamine | - | - |
| OA-4900 | (S)-Indoline-2-carboxylic acid (R)-1-(α-naphthyl)ethylamine | - | - |
| OA-5000 | (D)-Penicillamine | OA-5000L | (L)-Penicillamine |
| OA-6000 | (L)-Tartaric acid (R)-1-(α-Naphthyl)ethylamine | OA-6000R | (D)-Tartaric acid (S)-1-(α-Naphthyl)ethylamine |
| OA-6100 | (L)-Tartaric acid, (S)-Valine (S)-1-(α-Naphthyl)ethylamine | OA-6100R | (D)-Tartaric acid, (R)-Valine (R)-1-(α-Naphthyl)ethylamine |
| OA-7000 | β-Cyclodextrin | - | - |
| OA-7100 | β-Cyclodextrin | - | - |
| OA-7500 | Methyl-β-cyclodexitrin | - | - |
| OA-7600 | Methyl-α-cyclodexitrin | - | - |
| OA-7700 | Acetyl-β-cyclodexitrin | - | - |
| OA-8000 | Chiral pseudo-crown ether | - | - |
Shodex™
| HPLC column | Chiral selector |
| ORpak CDBS-453 | β-Cyclodextrin derivative |
| ORpak CRX-853 | L-amino acid derivative |
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