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Lipak
Lipak columns from SIELC are mixed-mode phases that have been specially developed for analysing lipids. The modification of the phase consists of a functionality for the reversed phase and a negatively charged group. This allows both polar and non-polar lipids to be retarded. This also enables the separation of complex lipid mixtures.
Applications
Mixed-mode analysis of phospholipids
Phosphopeptides are important biomolecules that play a crucial role in cell structure. This makes the analysis of phosphopeptides essential for lipidomics research. Due to different structures and fatty acid lengths, these compounds are difficult to analyse.
Peak identities
1. 18:0 Lyso PG 2. 16:0 Lyso PC 3. 18:0 Lyso PC 4. PLPC 5. cholesterol 6. DISIS 7. DPPC 8. DSPC 9. DIOPS 10. DSPG 11. DEPC
Test conditions
Column: Lipak 150x4.6mm, 5µm, 100A (LP-46.150.0510)
Mobile phase: Gradient methanol - 90-100% in 20 min, 10 min hold
Buffer: AmFm 10mM, FA 0.05%
Flow rate: 1.0 mL/min
Detection: ELSD
Mixed-mode separation of triglycerides
Triglycerides are the primary form of fat in our bodies. Triglycerides consist of three fatty acids bound to a glycerol backbone. The structure influences their physical and chemical properties, making it important to analyse them in the field of nutrition and clinical diagnostics.
Peak identities
Olive oil: Triolein (OOO): 40-60%; Oleic-Oleic-Palmitic (OOP): 10-20%; Oleic-Oleic-Linoleic (OOL): 15-20%; Oleic-Linoleic-Palmitic (OLP): 5-10%
Sunflower oil: Trilinolein (LLL): 50-65%; Linoleic-Oleic-Linoleic (LOL): 10-20%; Oleic-Linoleic-Linoleic (OLL): 5-15%
Maize oil: trilinolein (LLL): 25-50%; linoleic-oleic-linoleic (LOL): 15-30%; oleic-linoleic-linoleic (OLL): 15-25%
Test conditions
Column: Lipak 50x4.6mm, 5µm 100A (LP-46.50.0510)
Mobile phase: Gradient methanol/ethanol/water - 90/0/10 - 50/50/0 in 10 min
Buffer: AmF 10mM, FA 0.05%
Flow rate: 2.0 mL/min
Detection: ELSD
Downloads
Brochures
Sielc Lipak Lipids Brochure
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