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OtaCLEAN
The OtaCLEAN series from LCTech comprises selective immunoaffinity columns for efficient sample preparation for the analysis of ochratoxins, in which the target analytes are bound to the IAC column and separated from the matrix. Subsequent elution yields ochratoxins ready for injection into the LC system. The OtaCLEAN SMART variant features a shorter column length, thereby reducing solvent consumption and the time required for sample preparation. It is also possible to connect several SMART columns in series (e.g. from the AflaCLEAN series) to purify different analytes simultaneously.
Technical Data
Product | Shelf life (from date of manufacture) | Storage temperature | Size | Loading capacity | Recoveries of Ochratoxin A | LOD (limit of detection) |
|---|---|---|---|---|---|---|
OtaCLEAN | 24 months | 4 °C – 30 °C | 3 μL | 200 ng ochratoxin A | >90% | 0.04 ng/mL |
OtaCLEAN SMART | 9 months | 4 °C – 8 °C | 3 cmb | 100 ng ochratoxin A | >80% | 0.01 ng/mL |
a: Capacity of the IAC column, b: Total length of the IAC column.
What makes LCTech’s SMART series stand out?
LCTech’s SMART-IAC columns are based on a compact column design for immunoaffinity-based sample purification. Compared to conventional IAC columns with a 3 mL capacity, both the required sample volume of the matrix and the consumption of solvents are reduced. This allows sample preparation to be carried out with lower resource requirements. Due to the reduced column volume, the overall processing time of the method is shortened, allowing sample series to be processed more efficiently. This is particularly advantageous in analytical applications with high sample throughput. In addition, the SMART series features standardised Luer connectors, allowing multiple SMART-IAC columns to be connected in series. This enables different immunoaffinity-based selectivities to be combined in a single sample preparation step, making it possible to purify different mycotoxins in parallel within a single workflow.
The reduction in the volume of matrix and solvent used does not impair recovery rates. Following sample preparation in accordance with the LCTech protocol, the mycotoxins are obtained in a ready-to-inject form and at a concentration comparable to that of conventional 3-mL IAC purification. The sensitivity of the subsequent HPLC analysis remains unchanged, whilst the total processing time is reduced to a quarter of the original time.
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