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The new HPLC-column Intrada Amino Acid manufactured by Imtakt, has been developed for the determination of amino acids exclusively for LC-MS applications. Available internal diameters are 1 mm, 2mm and 3 mm and lengths 10 mm to 250 mm. A specially modified silica gel with particle size of 3 µm serves as stationary phase.
| Length (mm) | Inner diameter 1 mm | Inner diameter 2 mm | Inner diameter 3 mm |
| 10 | - | WAA20 | WAA30 |
| 20 | - | WAA29 | WAA39 |
| 30 | WAA11 | WAA21 | WAA31 |
| 50 | WAA12 | WAA22 | WAA32 |
| 75 | WAA13 | WAA23 | WAA33 |
| 100 | WAA14 | WAA24 | WAA34 |
| 150 | WAA15 | WAA25 | WAA35 |
| 250 | WAA16 | WAA26 | WAA36 |
Determination of 20 amino acids
Trp = tryptophan
Phe = phenylalanine
Tyr = tyrosine
Met = methionine
Leu = leucine , Ile= isoleucine
Val = valine
Glu = glutamic acid
Pro = proline
Thr = threonine
Asp = aspartic acid
Ala = alanine
Ser = serine
Gln = glutamine , Lys = lysine
Gly = glycine
Asn = asparagine
Cys = cysteine
His = hystidine
Arg = arginine
Test Conditions:
Column: Intarda Amino Acid 50 x 3 mm
Mobile Phase A: Acetonitril /Tetrahydrofuran /25mM Ammoniumformiat /Ameisensäure = 9 /75 /16 /0.3
Mobile Phase B: Acetonitril /100mM Ammoniumformiat = 20 / 80
Gradient:
| Time (min) | %B |
| 0 | 0 |
| 2.5 | 17 |
| 6.5 | 100 |
Flow rate: 0.6 mL/min
Temperature: 35°C
Injection volume: 5 µL
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