Checkout using your account
Checkout as a new customer
Creating an account has many benefits:
- See order and shipping status
- Track order history
- Check out faster
Proteomix HIC-ADC DARx Butyl
Sepax’s Proteomix HIC-ADC DARx Butyl columns have been specifically developed for the high-resolution analysis of antibody-drug conjugates (ADCs). The HIC columns enable rapid and reproducible DAR (drug-to-antibody ratio) profiling under LC-MS-compatible conditions and are ideal for high-throughput ADC screening, process development and quality control.
Hydrophobic interaction chromatography (HIC) enables reliable separation and quantitative analysis of DAR0–DAR8 species. The special butyl phase on non-porous PS/DVB particles combines high resolution with low backpressure and excellent robustness.
MZ Analytical Technology supports you in selecting suitable HIC columns for ADC characterisation, mAb analysis and biopharmaceutical LC-MS applications.
Technical Data
| Phase | Modification | Matrix | Pore size |
| Proteomix HIC-ADC DARx Butyl | C4 | PS/DVB with hydrophilic coating | non-porous |
Advantages of the Sepax Proteomix HIC-ADX DARx Butyl columns
High lot-to-lot reproducibility
High reproducibility of consecutive injections under high-throughput LC-MS conditions
High stability under high-throughput conditions
When would you benefit from the Proteomix HIC-ADC DARx Butyl?
High resolution at high flow rates
Resolution remains high even at higher flow rates, and the DAR values observed remain comparable. Due to the low backpressure of the Proteomix HIC-ADC DARx Butyl column, it can be operated with standard HPLC systems at higher flow rates.
Test conditions
Column: Proteomix HIC-ADC DARx Butyl, 5 µm, 100 × 4.6 mm
Mobile phase A: 1.5 M ammonium tartrate (pH 7.0)
Mobile phase B: 25 mM ammonium tartrate with 25% isopropanol (pH 7.0)
Gradient:
| Time / min | %A | %B |
| 0 | 100 | 0 |
| 12 | 0 | 100 |
| 17 | 0 | 100 |
| 22 | 100 | 0 |
Flow rate: 0.3, 0.5, 0.8 mL/min
Sample: SigmaMAb Antibody-Drug Conjugate (ADC) Mimic (1 mg/mL), Injection: 8 µg
Detection: UV 280 nm
Temperature: 25 °C
High resolution with fast gradients
Thanks to the short columns and high resolution, the various DAR components can be separated in under 3 minutes. This makes it ideal for routine high-throughput analysis of DAR.
Test conditions
Column: Proteomix HIC-ADC DARx Butyl, 5 µm, 35 × 4.6 mm
Mobile phase A: 1.5 M ammonium tartrate (pH 7.0)
Mobile phase B: 25 mM ammonium tartrate with 25% isopropanol (pH 7.0)
Gradient (3 minutes):
| Time / min | %A | %B |
| 0 | 100 | 0 |
| 3 | 0 | 100 |
| 5 | 0 | 100 |
| 8 | 100 | 0 |
Gradient (2 minutes):
| Time / min | %A | %B |
| 0 | 100 | 0 |
| 2 | 0 | 100 |
| 4 | 0 | 100 |
| 6.5 | 100 | 0 |
Flow rate: 1.5, 2.0 mL/min
Sample: SigmaMAb Antibody-Drug Conjugate (ADC) Mimic (1 mg/mL), Injection: 8 µg
Detection: UV 280 nm
Downloads
Sepax Proteomix HIC-ADC DARx Butyl Brochure
The right column for you - we will be happy to support you individually
Competent consultants are always at your side. Write a message to our consultants, we will get back to you and give you individual support.
You will find:
Application support
Availability and delivery times
Price information with individual conditions
Certificates of analysis, MSDS, data sheets