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Proteomix HIC-ADC DARx Butyl
Proteomix HIC-ADC DARx Butyl columns from Seapx have been specially developed for the reliable high-throughput analysis of antibody-drug conjugates (ADCs) using hydrophobic interaction chromatography (HIC). The drug-antibody ratio (ADC) can be determined with high resolution using the Proteomix HIC-ADC DARx Butyl. Furthermore, the column is MS-compatible, meaning integration into the LC-MS workflow is straightforward. Due to the small column sizes, very little sample is required and low concentrations can also be detected.
Technical Data
| Phase | Modification | Matrix | Pore size |
| Proteomix HIC-ADC DARx Butyl | C4 | PS/DVB with hydrophilic coating | non-porous |
What makes the Proteomix HIC-ADC DARx Butyl stand out?
High lot-to-lot reproducibility
High reproducibility of consecutive injections under high-throughput LC-MS conditions
High stability under high-throughput conditions
When would you benefit from the Proteomix HIC-ADC DARx Butyl?
High resolution at high flow rates
Resolution remains high even at higher flow rates, and the DAR values observed remain comparable. Due to the low backpressure of the Proteomix HIC-ADC DARx Butyl column, it can be operated with standard HPLC systems at higher flow rates.
Test conditions
Column: Proteomix HIC-ADC DARx Butyl, 5 µm, 100 × 4.6 mm
Mobile phase A: 1.5 M ammonium tartrate (pH 7.0)
Mobile phase B: 25 mM ammonium tartrate with 25% isopropanol (pH 7.0)
Gradient:
| Time / min | %A | %B |
| 0 | 100 | 0 |
| 12 | 0 | 100 |
| 17 | 0 | 100 |
| 22 | 100 | 0 |
Flow rate: 0.3, 0.5, 0.8 mL/min
Sample: SigmaMAb Antibody-Drug Conjugate (ADC) Mimic (1 mg/mL), Injection: 8 µg
Detection: UV 280 nm
Temperature: 25 °C
High resolution with fast gradients
Thanks to the short columns and high resolution, the various DAR components can be separated in under 3 minutes. This makes it ideal for routine high-throughput analysis of DAR.
Test conditions
Column: Proteomix HIC-ADC DARx Butyl, 5 µm, 35 × 4.6 mm
Mobile phase A: 1.5 M ammonium tartrate (pH 7.0)
Mobile phase B: 25 mM ammonium tartrate with 25% isopropanol (pH 7.0)
Gradient (3 minutes):
| Time / min | %A | %B |
| 0 | 100 | 0 |
| 3 | 0 | 100 |
| 5 | 0 | 100 |
| 8 | 100 | 0 |
Gradient (2 minutes):
| Time / min | %A | %B |
| 0 | 100 | 0 |
| 2 | 0 | 100 |
| 4 | 0 | 100 |
| 6.5 | 100 | 0 |
Flow rate: 1.5, 2.0 mL/min
Sample: SigmaMAb Antibody-Drug Conjugate (ADC) Mimic (1 mg/mL), Injection: 8 µg
Detection: UV 280 nm
The right column for you - we will be happy to support you individually
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