Ion Exchange Chromatography

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Ion Exchange Chromatography

Ion exchange chromatography (IEX) is used to separate and analyse charged or ionisable molecules. A distinction is made between anion and cation exchangers, and also between strong and weak exchangers.

SAX: Strong Anion Exchanger
WAX: Weak Anion Exchanger
SCX: Strong Cation Exchanger
WCX: Weak Cation Exchanger

Ion exchange chromatography is often used as a bioseparation technique for proteins and antibodies. Proteins are separated according to their net or surface charge, which is dependent on the pH value and the ionic strength of the mobile phase. Other areas of application include oligonucleotides, sugars, RNA and DNA molecules and more.

Ion chromatography and ion exclusion chromatography are two special cases of ion exchange chromatography with their own column technologies for specific applications.

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InertSep PSA 1000g

5010-69153

€1,050.00

Product Type: SPE Bulk Sorbent Amount: 1 kg Particle Size: 60 µm Pore Size: 60 Å Chemistry: Weak anion-exchange Manufacturer: GL Sciences Delivery Time: approx. 7 days Endcapping: non endcapped Carbon Load: 11.5 % Bet Surface Area [m2/g]: 450.0 Mode of Separation: Ion Exchange Chromatography Matrix: Silica Particle Morphology: fully porous pH Minimum: 2.0 pH Maximum: 8.0

InertSep WAX Micro Elution 96WP 2mg/400uL 1pk

5010-66824

€390.00

Product Type: Well Plates Particle Size: 30 µm Pore Size: 90 Å Chemistry: Weak anion-exchange Manufacturer: GL Sciences Delivery Time: approx. 7 days Bet Surface Area [m2/g]: 520.0 Base Material: Styrene-divinylbenzene Bed Mass [mg]: 2 mg Mode of Separation: Ion Exchange Chromatography Matrix: Polymer Particle Morphology: fully porous pH Minimum: 1.0 pH Maximum: 14.0 Volume: 400 µl

InertSep MAX Micro Elution 96WP 2mg/400uL 1pk

5010-66823

€390.00

Product Type: Well Plates Particle Size: 30 µm Pore Size: 90 Å Chemistry: Strong anion-exchange Manufacturer: GL Sciences Delivery Time: approx. 7 days Bet Surface Area [m2/g]: 520.0 Base Material: Styrene-divinylbenzene Bed Mass [mg]: 2 mg Mode of Separation: Ion Exchange Chromatography Matrix: Polymer Particle Morphology: fully porous pH Minimum: 1.0 pH Maximum: 14.0 Volume: 400 µl

InertSep WCX Micro Elution 96WP 2mg/400uL 1pk

5010-66822

€390.00

InertSep MCX Micro Elution 96WP 2mg/400uL 1pk

5010-66821

€390.00

Product Type: Well Plates Particle Size: 30 µm Chemistry: Strong cation-exchange Manufacturer: GL Sciences Delivery Time: approx. 7 days Bet Surface Area [m2/g]: 520.0 Base Material: Styrene-divinylbenzene Bed Mass [mg]: 2 mg Mode of Separation: Ion Exchange Chromatography Matrix: Polymer Particle Morphology: fully porous pH Minimum: 1.0 pH Maximum: 14.0 Volume: 400 µl

InertSep Oligo 96WP 2mg/400uL 1pk

5010-66811

€330.00

Product Type: Well Plates Chemistry: Weak anion-exchange Manufacturer: GL Sciences Delivery Time: approx. 7 days Bed Mass [mg]: 2 mg Mode of Separation: Ion Exchange Chromatography Volume: 400 µl

InertSep Oligo 96WP 25mg/2mL(Square) 1pk

5010-66810

€370.00

Product Type: Well Plates Chemistry: Weak anion-exchange Manufacturer: GL Sciences Delivery Time: approx. 7 days Bed Mass [mg]: 25 mg Mode of Separation: Ion Exchange Chromatography Volume: 2 ml

InertSep SAX 100mg/3mL 50/pk

5010-61656

€252.00

Product Type: SPE Cartridges Particle Size: 45 µm Pore Size: 60 Å Chemistry: Strong anion-exchange Manufacturer: GL Sciences Delivery Time: approx. 7 days Endcapping: non endcapped Carbon Load: 7.0 % Bet Surface Area [m2/g]: 450.0 Bed Mass [mg]: 100 mg Mode of Separation: Ion Exchange Chromatography Matrix: Silica Particle Morphology: fully porous pH Minimum: 2.0 pH Maximum: 8.0 Volume: 3 ml

Inertsil CX 5µm, HPLC-Column 125x4.6mm HPLC Column

5020-90697

€591.00

Product Type: HPLC Column Length: 125 mm Internal Diameter: 4.6 mm Particle Size: 5 µm Pore Size: 100 Å Chemistry: Strong cation-exchange USP Class: L9 Manufacturer: GL Sciences Delivery Time: approx. 7 days Column Type: Analytical Column Endcapping: non endcapped Carbon Load: 14.0 % Bet Surface Area [m2/g]: 450.0 Mode of Separation: Ion Exchange Chromatography Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, fully porous pH Minimum: 2.0 pH Maximum: 7.5 Technology: HPLC

InertSphere Sugar-1 5µm, Guard Kit 10x4.0mm HPLC Guard Kits

5020-19098

€412.00

Product Type: HPLC Guard Kits Length: 10 mm Internal Diameter: 4.0 mm Particle Size: 5 µm Chemistry: Strong anion-exchange Manufacturer: GL Sciences Delivery Time: approx. 7 days Base Material: Styrene-divinylbenzene Mode of Separation: Ion Exchange Chromatography Hardware Type: Cartridges Matrix: Polymer pH Minimum: 2.0 pH Maximum: 14.0 Pressure Stability: 150 bar Technology: HPLC

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Technical Data

An elution sequence for ion exchange chromatography (IEX) is often difficult to predict because the elution or retention of molecules depends on many parameters. Important parameters include the type of ion exchanger used, the pH value, the ionic strength and the type of counterion in the mobile phase, as well as the electronic properties of the analytes.

The surface of the stationary phases carry ionic groups, which are neutralised to the outside by mobile counterions of the mobile phase.

Representation of the stationary phase of an ion exchange material with counterions from the mobile phase

The analyte molecules compete with each other and with the counterions present in the mobile phase for a place on the surface of the stationary phase. As a rule, more strongly charged analyte molecules are also more strongly retarded than those that only have weak charges or are neutral.

Representation of the interaction of an analyte with the stationary phase of an ion exchange phase

Anion exchangers carry positive charges on the surface, e.g. quaternary or primary ammonium groups, in order to bind anions. Cation exchangers therefore carry negative charges on the surface,
e.g. sulphonates or carboxylates, in order to bind cations (see figure for examples).

Representation of different ion exchange groups for stationary phases

How do I choose the right ion exchange column?

The selection of a suitable ion exchange column is very complex and depends on many parameters, such as the pH value, the ionic strength and the electronic properties of the analytes.

First of all, however, it is crucial which type of ions are to be analysed (anions or cations). Accordingly, there are strong and weak ion exchange columns. Strong ion exchange columns have a charge independent of the pH value used. This means that retention remains stable even if the pH value changes (depending on the analyte). With weak ion exchange columns, the pH value changes the ionisation of the stationary phase and thus the properties. Retention can therefore be controlled via the pH value.

As elution is difficult to predict due to the large number of different parameters, it is advisable to research the literature or various manufacturers before selecting a column. We will be happy to help you select the right column. Please contact us!

Ion exchange chromatography manufacturers and columns

1. Columns for cation exchange chromatography

Manufacturer	Name	Base material	Modification	Pore size	Particle size	pH range
Tosoh Bioscience	TSKgel CM-2SW	Silica gel	Carboxymethyl (Na⁺)	125 Å	5 µm	2.0-7.5
	TSKgel CM-3SW	Silica gel	Carboxymethyl (Na⁺)	250 Å	10 µm	2.0-7.5
	TSKgel SP-2SW	Silica gel	Sulfopropyl (Na⁺)	125 Å	5 µm	2.0-7.5
	TSKgel CM-5PW	Polymethacrylates	Carboxymethyl (Na⁺)	1000 Å	10 & 13 µm	2.0-12.0
	TSKgel SP-5PW	Polymethacrylates	Sulfopropyl (Na⁺)	1000 Å	10, 13 & 20 µm	2.0-12.0
	TSKgel SP-NPR	Polymethacrylates	Sulfopropyl (Na⁺)	non-porous	2.5 µm	2.0-12.0
	TSKgel SP-STAT	Polymethacrylates	Sulfopropyl (Na⁺)	non-porous	7 & 10 µm	3.0-10.0
	TSKgel CM-STAT	Polymethacrylates	Carboxymethyl (Na⁺)	non-porous	7 & 10 µm	3.0-10.0
	TSKgel Bioassist S PEEK	Polymethacrylates	Sulfopropyl (Na⁺)	~1300 Å	7 & 13 µm	2.0-12.0
	TSKgel SCX (Na+)	PS/DVB	Sulphonic acid (Na⁺)	60 Å	5 µm	1.0-14.0
	TSKgel SCX (H+)	PS/DVB	Sulphonic acid (H⁺)	60 Å	5 µm	1.0-14.0
Shodex	IEC SP-825	Polymethacrylates	Sulfopropyl	5000 Å	8 µm	2.0-12.0
	IEC SP-2025	Polymethacrylates	Sulfopropyl	5000 Å	20 µm	2.0-12.0
	IEC SP-FT 4A	Polymethacrylates	Sulfopropyl	non-porous	2.7 µm	2.0-12.0
	IEC CM-825	Polymethacrylates	Carboxymethyl	5000 Å	8 µm	2.0-12.0
	IEC CM-2025	Polymethacrylates	Carboxymethyl	5000 Å	20 µm	2.0-12.0
	Asahipak ES-502C 7C	Polymethacrylates	Carboxymethyl	2000 Å	9 µm	2.0-12.0
	Asahipak ES-502C 20C	Polymethacrylates	Carboxymethyl	2000 Å	13 µm	2.0-12.0
	CXpak P-421S	PS/DVB	Sulphonic acid (Na⁺)	N/A	6 µm	3.0-14.0
	CXpak P-G	PS/DVB	Sulphonic acid (Na⁺)	N/A	6 µm	3.0-14.0
Sepax	Proteomix SCX	PS/DVB with neutral hydrophilic polymer layer	Sulphonate	non-porous	1.7, 3, 5 & 10 µm	2.0-12.0
	Proteomix WCX	PS/DVB with neutral hydrophilic polymer layer	Carboxylates	non-porous	1.7, 3, 5 & 10 µm	2.0-12.0
	Antibodix WCX	PS/DVB with neutral hydrophilic polymer layer	Carboxylates	non-porous	1.7, 3, 5 & 10 µm	2.0-12.0
GL Sciences	InertSil CX	Silica Gel	Benzenesulfonic acid	100 Å	5 µm	2.0-7.5
Macherey nail	Nucleogel SCX	Polymethacrylates	Sulphonates	1000 Å	8 µm	1.0-13.0
Separation Methods Technologies	SMT SCX	Silica Gel	Sulfonic acid	100 Å, 300 Å	5 & 10 µm	2.0-7.5
	SMT WCX	Silica gel	Carboxylic acid	100 Å, 300 Å	5 & 10 µm	2.0-7.5
Supelco	Discovery Bio PolyMA-SCX	Polymethacrylates	Sulfopropyl (Na⁺)	1000 Å	5 µm	1.0-13.0
Thermo Scientific	BioBasic SCX	Silica Gel	Sulfonic acid	300 Å	5 µm	2.0-7.5
	ProPac SCX-10	Ethylvinylbenzene crosslinked with 55% divinylbenzene	Sulfonic acid	non-porous	10 µm	2.0-12.0
	ProPac SCX-20	Ethylvinylbenzene crosslinked with 55% divinylbenzene	Sulfonic acid	non-porous	10 µm	2.0-12.0
	ProPac WCX-10	Ethylvinylbenzene crosslinked with 55% divinylbenzene	Carboxylic acid	non-porous	10 µm	2.0-12.0
	ProPac Elite WCX	DVB particles with hydrophilic coating	Carboxylic acid	non-porous	5 µm	2.0-12.0
	ProSwift SCX-1S	Polymethacrylate	Sulphonic acid	N/A	monolithic	2.0-12.0
	ProSwift WCX-1S	Polymethacrylate	Carboxylic acid	N/A	monolithic	2.0-12.0
	MAbPac SCX-10	Polydivinylbenzene	Sulfonic acid	non-porous	3, 5, & 10 µm	2.0-12.0

2. Columns for anion exchange chromatography

Manufacturer	Name	Base material	Modification	Pore size	Particle size	pH range
Tosoh Bioscience	TSKgel BioAssist Q	Polymethacrylates	Polyamines (Cl^-)	~4000 Å	10 & 13 µm	2.0-12.0
	TSKgel SuperQ-5PW	Polymethacrylates	Trimethyl-amino (Cl^-)	1000 Å	10 & 13 µm	2.0-12.0
	TSKgel DEAE-5PW	Polymethacrylates	Diethylaminoethyl (Cl^-)	1000 Å	10, 13 & 20 µm	2.0-12.0
	TSKgel Q-STAT	Polymethacrylates	Trimethyl-amino (Cl^-)	non-porous	7 & 10 µm	3.0-10.0
	TSKgel DNA-STAT	Polymethacrylates	Trimethyl-amino (Cl^-)	non-porous	5 µm	3.0-10.0
	TSKgel DEAE-NPR	Polymethacrylates	Diethylaminoethyl (Cl^-)	non-porous	2.5 µm	2.0-12.0
	TSKgel DNA-NPR	Polymethacrylate	Proprietary (ClO₄^-)	non-porous	2.5 µm	2.0-12.0
	TSKgel DEAE-2SW	Silica gel	Diethylaminoethyl (H₂PO₄^-)	125 Å	5 µm	2.0-7.5
	TSKgel DEAE-3SW	Silica gel	Diethylaminoethyl (Cl^-)	250 Å	10 µm	2.0-7.5
	TSKgel SUGAR AXI	PS/DVB	Trimethyl-amino (HBO₃^-)	60 Å	8 µm	1.0-14.0
	TSKgel SUGAR AXG	PS/DVB	Trimethyl-amino (HBO₃^-)	60 Å	10 µm	1.0-14.0
	TSKgel SAX	PS/DVB	Trimethyl-amino (Cl^-)	60 Å	5 µm	1.0-14.0
Shodex	IEC QA-825	Polymethacrylate	Quat. Ammonium	5000 Å	12 µm	2.0-12.0
	IEC QA-2025	Polymethacrylates	Quat. Ammonium	5000 Å	20 µm	2.0-12.0
	IEC DEAE-825	Polymethacrylates	Diethylaminoethyl	5000 Å	8 µm	2.0-12.0
	IEC DEAE-2025	Polymethacrylates	Diethylaminoethyl	5000 Å	20 µm	2.0-12.0
	AXpak WA-624	Polymethacrylates	Diethylaminoethyl	2000 Å	10 µm	3.0-12.0
	Asahipak ES-502N 7C	Polyvinyl alcohol	Diethylaminoethyl	2000 Å	9 µm	2.0-12.0
	Asahipak ES-502N 20C	Polyvinyl alcohol	Diethylaminoethyl	2000 Å	13 µm	2.0-12.0
Sepax	Proteomix SAX	PS/DVB with neutral hydrophilic polymer layer	Quat. Ammonium	non-porous	1.7, 3, 5 & 10 µm	2.0-12.0
	Proteomix WAX	PS/DVB with neutral hydrophilic polymer layer	Diethylaminoethyl	non-porous	1.7, 3, 5 & 10 µm	2.0-12.0
	Glycomix SAX	Hydrophilic polymer	Quat. Ammonium	N/A	N/A	2.0-12.0
GL Sciences	InertSil AX	Silica Gel	Diethylaminopropyl	100 Å	5 µm	2.0-7.5
Macherey nail	Nucleogen DEAE	Silica gel	Diethylaminoethyl	60, 500, 4000 Å	7 µm	2.0-7.5
	Nucleogel SAX	Quaternised PEI	Quat. Ammonium	1000 Å	8 µm	1.0-13.0
Separation Methods Technologies	SMT SAX	Silica Gel	Quat. Ammonium	100, 300 Å	5 & 10 µm	2.0-7.5
	SMT WAX	Silica gel	Polyethyleneimine	100, 300 Å	5 & 10 µm	2.0-7.5
Supelco	Discovery Bio PolyMA-WAX	Polymethacrylates	Diethylaminoethyl	1000 Å	5 µm	2.0-11.0
Thermo Scientific	Hypersil Gold AX	Silica Gel	Polyethyleneimine	175 Å	1.9, 3 & 5 µm	2.0-8.0
	HyperSil Gold SAX	Silica Gel	Quat. Ammonium	175 Å	1.9, 3 & 5 µm	2.0-8.0
	BioBasic AX	Silica Gel	Polyethyleneimine	300 Å	5 µm	2.0-8.0
	ProPac SAX-10	Ethylvinylbenzene crosslinked with 55% divinylbenzene	Quat. Ammonium	non-porous	10 µm	2.0-12.0
	ProPac WAX-10	Ethylvinylbenzene crosslinked with 55% divinylbenzene	Tertiary amines	non-porous	10 µm	2.0-12.0
	ProSwift SAX-1S	Polymethacrylate	Quat. Ammonium	N/A	monolithic	2.0-12.0
	ProSwift WAX-1S	polymethacrylates	Diethylaminoethyl	N/A	monolithic	2.0-12.0
	DNAPac PA100	Pellicular polymer beads	Quat. Ammonium	non-porous	13 µm	2.0-12.5
	DNAPac PA200	Pellicular polymer beads	Quat. Ammonium	non-porous	8 µm	2.0-12.5
	DNAPac PA200RS	Pellicular polymer beads	Quat. Ammonium	non-porous	4 µm	2.0-12.5
	DNASwift SAX-1S	Polymethacrylate	Quat. Ammonium	N/A	monolithic	6.0-12.4

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