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SurePac
SurePac HPLC columns from Thermo Scientific are designed for bio-LC. The SurePac Oligo RP MDi and SurePac Protein RP MDi columns are based on a polymer utilising monodisperse particle technology (MDi) and also feature inert hardware. The monodisperse particles ensure excellent reproducibility of the HPLC columns. Furthermore, these columns demonstrate high efficiency and consistent performance. The bio-inert hardware enables higher sample recovery and more reproducible results.
SurePac Oligo RP MDi has been specifically developed for the analysis of therapeutic oligonucleotides and nucleic acids of all sizes. As a result, the HPLC column demonstrates excellent resolution of truncated proteins and related impurities. The high pH stability (2.0–12.0) and high temperature stability (up to 90°C) make this column ideal for oligonucleotide analysis. This makes the column ideal for the biopharmaceutical industry, oligo development and all laboratories that routinely analyse oligonucleotides.
SurePac Protein RP MDi has been specifically designed for the analysis of proteins, monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs). This HPLC column is characterised by high protein resolution and high thermal and chemical stability.
Technical Data
What are the advantages of the SurePac Oligo RP MDi?
High lot-to-lot reproducibility in DNA analysis
Column: SurePac Oligo RP MDi 50x2.1mm
Sample: 8-combo ssDNA mix
Robust and stable retention after more than 1,000 injections
Column: SurePac Oligo RP MDi 50x2.1mm
Sample: 8-combo ssDNA mix
Applications
When can you benefit from the SurePac Oligo RP MDi?
A faster method thanks to high temperature stability
Column: SurePac Oligo RP MDi 50x2.1mm
Sample: 8-combo ssDNA mix
Mobile phase A: 0.1 M HAA
Mobile phase B: acetonitrile
Gradient:
| Time | %A | %B |
| 0 | 89 | 11 |
| 9 | 50 | 50 |
| 9.1 | 25 | 75 |
| 11 | 25 | 75 |
| 11.1 | 89 | 11 |
| 16 | 89 | 11 |
Flow rate: 0.4 mL/min
Injection volume: 2.0 µL
Detection: UV (260 nm)
High resolution for oligonucleotides around 100 nt
Column: SurePac Oligo RP MDi, 50 × 2.1 mm
Sample: 98–100-mer ssDNA mix (20, 20 and 40 µg/mL)
Mobile phase A: 0.1 M TEAA
Mobile phase B: acetonitrile
Gradient:
- 8–18% in 10 min for Vendor B
- 6–16% in 10 min for all other columns
Temperature: 60 °C
Flow rate: 0.4 mL/min
Injection volume:
- 2.5 µL for 50 × 2.1 mm
- 5.0 µL for 100 × 2.1 mm
Detection: UV (260 nm)
High resolution for nucleic acids up to 1353 bp
Column: SurePac Oligo RP MDi, 50 × 2.1 mm
Sample: dsDNA (100 µg/mL)
Mobile phase A: 0.1 M TEAA
Mobile phase B: acetonitrile
Gradient:
- 9–18% in 10 min for Vendor B
- 11–20% in 10 minutes for all other columns
Temperature: 50 °C
Flow rate: 0.4 mL/min
Injection volume:
- 5 µL for 50 × 2.1 mm
- 10 µL for 100 × 2.1 mm
Detection: UV (260 nm)
Downloads
Brochures
Thermo SurePac Oligo RP MDi High resolution bio-inert HPLC Columns for Oligonucleotide Analysis
Thermo SurePac Oligo RP MDi Reversed Phase Analysis of Nucleic Acids
Technical Support
Thermo SurePac Oligo RP MDi Product Manual
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