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Ion Exclusion Chromatography
Ion exclusion chromatography is a specialised technique of Ion exchange chromatography which is often used for analysing sugars, organic acids and alcohols.
Technical Data
A completely sulphonated cation exchanger is often used as the stationary phase. A hydrate shell is formed around the sulphonic acid groups using low-concentration, strong aqueous acids as the mobile phase. This is bounded by an (imaginary) partially negatively charged membrane, which is also referred to in the literature as a Donnan membrane. The stationary phase is therefore only accessible to uncharged, non-dissociated molecules such as water. Analytes with a strongly acidic character are excluded, as these are still partially deprotonated under the conditions of the mobile phase and are therefore repelled by the Donnan membrane.
Excluded ions cannot penetrate the pore system of the stationary phase and are the first to be eluted. Weak acids, on the other hand, are protonated in the solvent, i.e. they are uncharged. They can therefore pass through the Donnan membrane and go to the surface of the stationary phase, where they are separated. Ion-exclusion chromatography is often used for the simultaneous separation of sugars, organic acids and alcohols, e.g. in fermentation analysis.
How do I choose the right ion exclusion column?
As the ion-exclusion columns are always sulphonated polymer columns that have a proton as a counter ion, the choice of modification is irrelevant in this case. The cross-linking of the polymer is much more important here.
The standard cross-linking of ion-exclusion columns is 8% and is suitable for many routine applications. However, if the cross-linking is changed, the size exclusion mechanism plays a greater role in addition to the ion exclusion mechanism. This tends to lead to higher resolutions for columns with lower cross-linking. One disadvantage, however, is the lower pressure stability, which results in lower flow rates and therefore longer run times.
Due to the simple mobile phase (water with sulphuric acid), it is hardly possible to optimise the separation using the mobile phase. This makes the choice of the right column even more important and is essentially dependent on your application. So-called retention tables help with the selection. The manufacturers of ion exclusion columns specify the retention of standard organic acids, alcohols and sugars to simplify the selection process. Some typical applications can also be found from various manufacturers. We will be happy to assist you in selecting the right column for you. Please contact us!
Manufacturers and columns of ion exclusion chromatography
Concise Separations offers a very wide range of high quality columns for ion exclusion chromatography. Materials with different degrees of cross-linking and particle sizes are available, covering a wide range of applications.
Shodex ion exclusion columns are proven columns for analysing organic acids, alcohols and carbohydrates.
Sepax Carbomix H is a column for ion exclusion chromatography.
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