Unison UK-C18 3µm,ODS,end-capped 50x2mm LxID

The Unison UK-C18 HPLC column 50x2.0mm, 3µm, carries an octadecyl modification (ODS, RP-18, C18) with polymeric methyl endcapping on fully porous high-purity silica with 13 nm pores and is assigned to USP class L1. The surface maintains retention even with fully aqueous eluents, so the retention loss occurring with many reversed phases is eliminated. Compared with the somewhat more hydrophobic Cadenza CD-C18, Unison UK-C18 retains polar substances more strongly, while preserving the spatial differentiation of stereoisomers. Applications reported include statins such as atorvastatin and rosuvastatin, nitrofuran antibiotics and hormones in veterinary medicines, ceftazidime, donepezil, entecavir, β-carboline alkaloids, sucrose fatty acid esters as well as OLED and dye-sensitised solar cell materials.

Features and Benefits

• Classic phase chemistries on high-purity silica
• Polymer end-capping for acid and alkali resistance
• Stable even with fully aqueous eluents
• Low carryover in LC-MS

Additional Notes

Unison applies its established phase chemistries to the brand’s high-purity, fully porous silica supports. Columns designated “UK” are the 3 µm versions, while those designated “US” are the 5 µm versions of the same phase with the same surface treatment. In addition, each chemistry is available in a pressure-resistant version rated for 500 bar, a version rated for 1000 bar, and a metal-free version with a PEEK-coated fluid path, each featuring the same packing material. The brand is designed for long columns, so even with 3 µm, high separation step numbers can be achieved without an UHPLC system. When transferring methods, retention, resolution, peak shape, and system pressure must be verified, as selectivity can vary between column chemistries even within the same USP class.

Legal Information

Unison is a product line of Imtakt Corporation, Kyoto, Japan. The product and phase names mentioned are part of the manufacturer's chromatography portfolio.