Intrada SEC 3µm, Diol-Silica for SEC 250x10mm LxID

The Intrada SEC HPLC column 250x10mm, 3µm, features a diol phase (Diol) on fully porous high-purity silica with 30 nm pores and separates by molecular size rather than by hydrophobicity. The neutral hydrophilic surface suppresses nonspecific interactions and permits both aqueous gel filtration and non-aqueous gel permeation up to about one megadalton, with an operating range of pH 1 to 8. Because the column can be operated with volatile eluents such as ammonium formate, it can be coupled directly to the mass spectrometer, unlike classical buffer systems. Monoclonal antibodies, serum albumin, enzymes, peptides, polysaccharides, nucleic acids, proteoglycans as well as polystyrene, polyethylene glycol and cellulose derivatives are demonstrated.

Features and Benefits

• Wide-pore supports with 30 nm pores
• Designed for proteins, peptides, and polymers
• Polymer end-capping in reverse-phase chromatography
• Size-exclusion chromatography in aqueous and non-aqueous systems
• Metal-free versions available

Additional Information

At Imtakt, Intrada is not a series of conventional reverse-phase columns, but rather a specialized line consisting of four columns, each designed for a clearly defined application. Intrada WP-RP is a wide-pore reverse-phase column for proteins and larger peptides, while Intrada SEC is a size-exclusion column with a diol phase for biomolecules and polymers. In addition, there are two columns for mass spectrometric analysis of small polar molecules: Intrada Amino Acid for intact amino acids without derivatization, and Intrada Organic Acid for organic acids in ion-exchange and normal-phase modes. Intrada WP-RP and Intrada SEC are both based on wide-pore pure silica with 30 nm pores, which allow even large molecules to access the surface. Additionally, metal-free versions are available with a PEEK-coated flow path and a stainless steel jacket for compounds whose peak shape is adversely affected by metallic surfaces.

Legal Information

Intrada is a product line of Imtakt Corporation, Kyoto, Japan. The product and phase names mentioned are part of the manufacturer's chromatography portfolio.