Intrada WP-RP 3µm 250x1.5mm (30nm reversed-phase end-capped)

The Intrada WP-RP HPLC column 250x1.5mm, 3µm, carries a reversed-phase alkyl ligand with polymeric endcapping and moderate surface polarity on wide-pore high-purity silica with 30 nm pores. The wide pores make the surface accessible to proteins and larger peptides that cannot enter the pores of narrow-pore reversed phases. Compared with wide-pore butyl phases, hydrophobic substances can be more readily eluted, which improves recovery of large molecules. Demonstrated are standard proteins of approximately 4 to 670 kilodaltons, including thyroglobulin, β-glucosidase, lactoferrin, haemoglobin and interferon, as well as amyloid-β peptide, porcine-skin collagen, bovine serum, cow milk proteins, phycocyanin from Spirulina, polyethylene glycol and hydroxypropylmethylcellulose.

Features and Benefits

• Wide-pore supports with 30 nm pores
• Designed for proteins, peptides, and polymers
• Polymer end-capping in reverse-phase chromatography
• Size-exclusion chromatography in aqueous and non-aqueous systems
• Metal-free versions available

Additional Information

At Imtakt, Intrada is not a series of conventional reverse-phase columns, but rather a specialized line consisting of four columns, each designed for a clearly defined application. Intrada WP-RP is a wide-pore reverse-phase column for proteins and larger peptides, while Intrada SEC is a size-exclusion column with a diol phase for biomolecules and polymers. In addition, there are two columns for mass spectrometric analysis of small polar molecules: Intrada Amino Acid for intact amino acids without derivatization, and Intrada Organic Acid for organic acids in ion-exchange and normal-phase modes. Intrada WP-RP and Intrada SEC are both based on wide-pore pure silica with 30 nm pores, which allow even large molecules to access the surface. Additionally, metal-free versions are available with a PEEK-coated flow path and a stainless steel jacket for compounds whose peak shape is adversely affected by metallic surfaces.

Legal Information

Intrada is a product line of Imtakt Corporation, Kyoto, Japan. The product and phase names mentioned are part of the manufacturer's chromatography portfolio.