IEC DEAE-2025 150x20mm Ion Exchange Preparative Column

The Shodex IEC DEAE-2025 preparative HPLC column 150x20mm, 20µm, is based on a large-pore, hydrophilic polyhydroxymethacrylate gel with diethylaminoethyl groups as a weak anion exchanger. The preparative packing bed transfers the pH-dependent DEAE selectivity to higher sample amounts and reduces unwanted hydrophobic interactions due to its hydrophilic surface. Shodex IEC DEAE-2025 is used for gentle purification of proteins, enzymes, nucleic acids and other anionic biomolecules.

Features and Benefits

• Strong and weak ion exchangers
• Anion and cation phases
• Large-pore materials for biomolecules
• Non-porous option for rapid separations
• Hydrophilic polymer backbones

Additional Notes

The IEX Series combines polyhydroxymethacrylate, polyvinyl alcohol, and styrene-divinylbenzene frameworks. Quaternary ammonium and sulfopropyl groups act as strong ion exchangers, while DEAE and carboxymethyl groups act as weak ion exchangers. Large-pore packing improves access for proteins and nucleic acids, while nonporous particles offer particularly short transport paths. The choice of phase depends on net charge, isoelectric point, molecular size, and the desired elution strategy.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. The IEX Series is a product line within the Shodex portfolio.