PROD,COL,PP,WCX-10,4X250MM,NOVARTIS

The ProPac WCX-10 HPLC column 250x4.0mm, 10µm, is based on non-porous polymer particles with weak cation-exchange groups. The pH-dependent negative charge enables selective and gentle binding of cationic proteins. Compared with SCX the weaker functionality can improve resolution near the isoelectric point. ProPac WCX-10 is used particularly for mAb charge variants, proteins and enzymes.

Features and Benefits

• Nonporous, surface-functionalized polymer phases
• Anion, cation, HIC, and affinity selectivities
• Rapid mass transport for biomolecules
• High resolution of charge and structural variants
• Analytical biopharmaceutical and carbohydrate methods

Additional Information

ProPac columns include polymer-based materials for charge-based variants, carbohydrates, hydrophobic variants, and metal affinity. Non-porous or thinly coated particles reduce diffusion paths and enable rapid biomolecule separations. For ion exchange, pH and ionic strength are optimized; for HIC, salt type and salt concentration are optimized; and for IMAC, metal ions and complexation conditions are optimized. Therefore, the respective ProPac modification cannot be interchanged based solely on the direction of exchange.

Legal Information

Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. ProPac refers to a product line within the Thermo Scientific chromatography portfolio.