ACE 1.7 HILIC Method Development UHPLC/HPLC Column Kit 100x3.0mm (HILIC-A, HILIC-B AND HILIC-N)

The Avantor ACE HILIC Method Development Kits HPLC-/UHPLC columns 100x3.0mm, 1.7µm, contain three complementary modifications for hydrophilic interaction chromatography. The kit contains Avantor ACE HILIC-A, Avantor ACE HILIC-B and Avantor ACE HILIC-N with the same length, same ID and same particle size, so that the selectivity of the individual phases can be directly compared. The Avantor ACE HILIC Method Development Kits are used primarily for polar, very polar and ionisable analytes when sufficient retention cannot be achieved in reversed phase. Typical applications are found in pharmaceutical analysis, bioanalysis, food analysis and environmental analysis, for example for nucleobases, nucleosides, organic acids, beta blockers or other hydrophilic compounds.

Features and Benefits

• Three proprietary HILIC phases for systematic method development
• Retention of polar and highly polar analytes outside the scope of conventional reverse-phase separation
• Distinct acidic, basic, and neutral selectivity profiles
• Ultra-inert silica base for reproducible HILIC separations
• Suitable for LC-MS-friendly mobile phases with a high organic content

Additional Notes

Avantor ACE HILIC uses ultra-inert silica particles with various proprietary polar surface chemistries. The three HILIC phases—HILIC-A, HILIC-B, and HILIC-N—were developed for systematic selectivity screening, as they represent different ionic and neutral interactions. In contrast to reverse-phase chromatography, retention in HILIC is typically determined by a water-rich layer on the polar stationary phase and mobile phases with a high organic content. Appropriate guard cartridges protect the sensitive HILIC method from matrix components and help ensure stable retention times.

Legal Notices

Avantor ACE is a registered trademark of the manufacturer Avantor, Inc., Radnor, USA.