STANDARD P-82, Pullulan calibration standard kit for GFC

The STANDARD P-82 contains eight pullulan standards, each weighing 0.2 g, numbered from P-5 to P-800. The nominal molar masses at peak maximum are 6,400 for P-5, 9,800 for P-10, 21,900 for P-20, 49,400 for P-50, 110,000 for P-100, 218,000 for P-200, 354,000 for P-400, and 692,000 for P-800. The distribution is narrow across all eight levels, as the Mw/Mn ratio remains between 1.05 and 1.30. The exact values vary from batch to batch and are listed for each shipment in the enclosed certificate of analysis. With eight standards spanning more than two orders of magnitude, a calibration curve can be plotted across the entire separation range of aqueous SEC columns. Potential applications include the determination of the molecular weight of polysaccharides and other water-soluble polymers, as well as the testing and comparison of aqueous SEC columns. A refractive index detector is suitable for detection, as pullulan absorbs only very weakly in the UV range. When unopened, the kit is usable for analytical purposes for two years from the date of the test certificate.

Features and Benefits

• Eight pullulan standards in one kit
• Narrow molecular weight distribution for each standard
• Molecular weights ranging from 6,400 to 692,000
• Rapidly and completely water-soluble
• No adsorption onto the packaging material

Additional Information

Pullulan is a water-soluble polysaccharide composed of maltotriose units linked via α-1,6 bonds to form a linear chain. It remains stable in solution because it does not tend to agglomerate or crystallize and carries no electrical charge. Since it has no charge, the molecules do not adhere to standard packing materials and elute in solution based solely on their size. This is precisely what makes pullulan the reference substance for aqueous systems in which polysaccharides and other polar polymers are separated. The calibration curve is generated by plotting the molecular weight at the peak maximum against the retention time under the same conditions under which the sample will later be run. The molecular weights determined in this way are relative values and are based on pullulan as the reference polymer.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo. The product and model names mentioned are part of the manufacturer's chromatography portfolio.