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The Asahipak ES-502N 7C HPLC column 100x7.5mm, 9µm, contains diethylaminoethyl groups as a weak anion exchanger on a macroporous polyvinyl alcohol gel. Negatively charged proteins and other biomolecules are bound via electrostatic interactions and eluted using salt or pH gradients. The hydrophilic PVA backbone minimises hydrophobic secondary interactions. Typical application areas of Asahipak ES-502N 7C are proteins, enzymes, nucleic acids and other anionic biomolecules.
The Asahipak product family is primarily based on porous polyvinyl alcohol particles. The hydrophilic polymer backbone is chemically stable and, compared to silica, produces only minimal undesirable interactions with basic compounds or biomolecules. Reverse-phase, HILIC, ion exchange, and size exclusion chromatography are supported through C18, C4, amino, and ion-exchange modifications, as well as defined pore sizes. Same-phase guard columns protect the packing bed from particles and highly retained matrix components.
Asahipak is a registered trademark of the manufacturer, Resonac Corporation, Tokyo, Japan.
| Flow Rate [ml/min] | 1.0 |
|---|---|
| Brand | Asahipak |
| Product Type | HPLC Column |
| Length | 100 mm |
| Internal Diameter | 7.5 mm |
| Particle Size | 9 µm |
| Chemistry Producers Term | ES |
| Pore Size | 2.000 Ã… |
| Country of Origin | Japan |
| Column Type | Semi-preparative Column |
| Solvent | 50 mM 1,3-Diaminopropane + 50 mM NaCl (pH 10.0) |
| Base Material | Polyvinylalcohol |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 12.0 |
| Temperature Minimum [°C] | 10 |
| Temperature Maximum [°C] | 50 |
| Mode of Separation | Ion Exchange Chromatography |
| Technology | HPLC |
| Matrix | Polymer |
| Number Theoretical Plates | ≥ 3,300 |
| Pressure Stability | 20 bar |