Asahipak GS-620 HQ 300x7.5mm Gel Filtration (GFC) Multimode

The Asahipak GS-620 HQ HPLC column 300x7.5mm, 7µm, contains a wide-pore polyvinyl alcohol gel with a nominal pore size of 7,000 Å. In SEC or multimode chromatography Asahipak GS-620 HQ typically covers a molecular weight range of approx. 10,000 to 800,000 Da for pullulan with an exclusion limit of approx. 1,000,000 Da. The large pore structure makes accessible proteins, polysaccharides, nucleic acids and other high-molecular-weight water-soluble macromolecules. Hydrophobic and ionic contributions can be controlled via the eluent composition. The stated molecular weight ranges are nominal, calibration-dependent orientation values.

Features and Benefits

• Hydrophilic polyvinyl alcohol matrix
• Multiple separation mechanisms available
• Broad solvent and pH compatibility
• Low nonspecific adsorption
• Analytical and preparative formats

Additional Information

The Asahipak product family is primarily based on porous polyvinyl alcohol particles. The hydrophilic polymer backbone is chemically stable and, compared to silica, produces only minimal undesirable interactions with basic compounds or biomolecules. Reverse-phase, HILIC, ion exchange, and size exclusion chromatography are supported through C18, C4, amino, and ion-exchange modifications, as well as defined pore sizes. Same-phase guard columns protect the packing bed from particles and highly retained matrix components.

Legal Information

Asahipak is a registered trademark of the manufacturer, Resonac Corporation, Tokyo, Japan.