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In the Asahipak GF-510 HQ SEC column 300x7.5mm, 5µm, a hydrophilic polyvinyl alcohol gel with a nominal pore size of 2,000 Å is contained. The separation range intended for Asahipak GF-510 HQ typically lies at approx. 5,000 to 200,000 Da (Pullulan) or 2,000 to 200,000 Da (PEG/PEO); this allows mainly polysaccharides, proteins and larger water-soluble polymers to be investigated according to size. The wide-pore matrix opens up significantly larger hydrodynamic volumes. The stated molecular weight ranges are nominal, calibration-dependent guideline values.
The Asahipak product family is primarily based on porous polyvinyl alcohol particles. The hydrophilic polymer backbone is chemically stable and, compared to silica, produces only minimal undesirable interactions with basic compounds or biomolecules. Reverse-phase, HILIC, ion exchange, and size exclusion chromatography are supported through C18, C4, amino, and ion-exchange modifications, as well as defined pore sizes. Same-phase guard columns protect the packing bed from particles and highly retained matrix components.
Asahipak is a registered trademark of the manufacturer, Resonac Corporation, Tokyo, Japan.
| Flow Rate [ml/min] | 0.4 to 0.6 |
|---|---|
| Brand | Asahipak |
| Product Type | SEC/GPC Columns |
| Length | 300 mm |
| Internal Diameter | 7.5 mm |
| Particle Size | 5 µm |
| Chemistry Producers Term | GF |
| Pore Size | 2.000 Ã… |
| Country of Origin | Japan |
| Column Type | Semi-preparative Column |
| Lower Molecular Weight | 2.000 (PEG/PEO), 5.000 (Pullulan) |
| Upper Molecular Weight | 200.000 (PEG/PEO), 200.000 (Pullulan) |
| Exclusion Limit | 300.000 (Pullulan) |
| Solvent | H2O/CH3OH = 70/30 |
| Base Material | Polyvinylalcohol |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 9.0 |
| Temperature Minimum [°C] | 4 |
| Temperature Maximum [°C] | 60 |
| Mode of Separation | SEC / Aqueous Size Exclusion Chromatography (GFC) |
| Technology | HPLC |
| Matrix | Polymer |
| Number Theoretical Plates | ≥ 19,000 |
| Pressure Stability | 65 bar |