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Asahipak GF-310 HQ, 7.5 x 300 mm, HPLC column
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Product Description
The Asahipak GF-310 HQ SEC column 300x7.5mm, 5µm, contains a hydrophilic polyvinyl alcohol gel with a nominal pore size of 400 Å. In aqueous and polar-organic size-exclusion chromatography, Asahipak GF-310 HQ covers a typical molecular weight range of approx. 300 to 30,000 Da (Pullulan) or 200 to 4,000 Da (PEG/PEO) and is particularly suitable for oligosaccharides, peptides and medium-sized water-soluble polymers. The medium pore size combines a wider measurement range with good resolution of small oligomers. The stated molecular weight ranges are nominal, calibration-dependent guideline values.
Features and Benefits
- Hydrophilic polyvinyl alcohol matrix
- Multiple separation mechanisms available
- Broad solvent and pH compatibility
- Low nonspecific adsorption
- Analytical and preparative formats
Additional Information
The Asahipak product family is primarily based on porous polyvinyl alcohol particles. The hydrophilic polymer backbone is chemically stable and, compared to silica, produces only minimal undesirable interactions with basic compounds or biomolecules. Reverse-phase, HILIC, ion exchange, and size exclusion chromatography are supported through C18, C4, amino, and ion-exchange modifications, as well as defined pore sizes. Same-phase guard columns protect the packing bed from particles and highly retained matrix components.
Legal Information
Asahipak is a registered trademark of the manufacturer, Resonac Corporation, Tokyo, Japan.
Product Specification
| Flow Rate [ml/min] | 0.4 to 0.6 |
|---|---|
| Brand | Asahipak |
| Product Type | SEC/GPC Columns |
| Length | 300 mm |
| Internal Diameter | 7.5 mm |
| Particle Size | 5 µm |
| Chemistry Producers Term | GF |
| Pore Size | 400 Ã… |
| Country of Origin | Japan |
| Column Type | Semi-preparative Column |
| Lower Molecular Weight | 200 (PEG/PEO), 300 (Pullulan) |
| Upper Molecular Weight | 4.000 (PEG/PEO), 30.000 (Pullulan) |
| Exclusion Limit | 40.000 (Pullulan) |
| Solvent | H2O/CH3OH = 70/30 |
| Base Material | Polyvinylalcohol |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 9.0 |
| Temperature Minimum [°C] | 4 |
| Temperature Maximum [°C] | 60 |
| Mode of Separation | SEC / Aqueous Size Exclusion Chromatography (GFC) |
| Technology | HPLC |
| Matrix | Polymer |
| Number Theoretical Plates | ≥ 19,000 |
| Pressure Stability | 70 bar |
