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The PROTEIN KW405-4F SEC column 300x4.6mm, 5µm, contains spherical silica with a hydrophilic polymer coating with a nominal pore size of 2.000 Å. The separation range intended for PROTEIN KW405-4F typically lies at approx. 200.000 to 20.000.000 Da; this allows size-based analysis of very large protein complexes, aggregates and virus-like particles. The particularly large pore structure is designed for high hydrodynamic radii. The ranges are nominal guidance values and depend on molecule shape, eluent and calibration.
The PROTEIN Series uses spherical, porous silica with a hydrophilic polymer coating. The coating reduces hydrophobic and ionic interactions, allowing size-based separation of proteins to take center stage. Different pore sizes target peptides, antibodies, aggregates, and very large protein complexes. Particle sizes and column IDs range from robust routine formats to highly efficient semi-micro and fast SEC columns.
Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. Protein Series is a product line within the Shodex portfolio.
| Flow Rate [ml/min] | 0.35 |
|---|---|
| Brand | PROTEIN |
| Product Type | SEC/GPC Columns |
| Length | 300 mm |
| Internal Diameter | 4.6 mm |
| Particle Size | 5 µm |
| Chemistry Producers Term | KW |
| Pore Size | 1.000 Ã… (10e3 Ã…) |
| USP Class | L20, L33, L59 |
| Country of Origin | Japan |
| Column Type | Analytical Column |
| Lower Molecular Weight | 100.000 (Pullulan), 200.000 (Protein) |
| Upper Molecular Weight | 700.000 (Pullulan), 20.000.000 (Protein) |
| Exclusion Limit | 20.000.000 (Protein), 1.300.000 (Pullulan) |
| Solvent | H2O |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 3.0 |
| pH Maximum | 7.5 |
| Temperature Minimum [°C] | 5 |
| Temperature Maximum [°C] | 45 |
| Mode of Separation | SEC / Aqueous Size Exclusion Chromatography (GFC) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |
| Number Theoretical Plates | ≥ 25,000 |
| Pressure Stability | 70 bar |