PROTEIN KW405-4F, 4.6 x 300 mm, HPLC column

Part Number: SD-F6989204
Manufacturer: Shodex - Resonac
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Delivery Time: approx. 5 days
Excl. 19% taxes, excl. Shipping costs
Packaging unit: 1 pc, Minimum order quantity: 1 pc
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Product Description

The PROTEIN KW405-4F SEC column 300x4.6mm, 5µm, contains spherical silica with a hydrophilic polymer coating with a nominal pore size of 2.000 Å. The separation range intended for PROTEIN KW405-4F typically lies at approx. 200.000 to 20.000.000 Da; this allows size-based analysis of very large protein complexes, aggregates and virus-like particles. The particularly large pore structure is designed for high hydrodynamic radii. The ranges are nominal guidance values and depend on molecule shape, eluent and calibration.

Features and Benefits

  • Silica-based Bio-SEC phases
  • Defined pores for different protein sizes
  • High efficiency and low adsorption
  • Analytical and semi-micro formats
  • Fast 1.9-µm version available

Additional Notes

The PROTEIN Series uses spherical, porous silica with a hydrophilic polymer coating. The coating reduces hydrophobic and ionic interactions, allowing size-based separation of proteins to take center stage. Different pore sizes target peptides, antibodies, aggregates, and very large protein complexes. Particle sizes and column IDs range from robust routine formats to highly efficient semi-micro and fast SEC columns.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. Protein Series is a product line within the Shodex portfolio.

Product Specification

Flow Rate [ml/min] 0.35
Brand PROTEIN
Product Type SEC/GPC Columns
Length 300 mm
Internal Diameter 4.6 mm
Particle Size 5 µm
Chemistry Producers Term KW
Pore Size 1.000 Ã… (10e3 Ã…)
USP Class L20, L33, L59
Country of Origin Japan
Column Type Analytical Column
Lower Molecular Weight 100.000 (Pullulan), 200.000 (Protein)
Upper Molecular Weight 700.000 (Pullulan), 20.000.000 (Protein)
Exclusion Limit 20.000.000 (Protein), 1.300.000 (Pullulan)
Solvent H2O
Hardware Material Stainless Steel
Hardware Type Ready-to-use Column
pH Minimum 3.0
pH Maximum 7.5
Temperature Minimum [°C] 5
Temperature Maximum [°C] 45
Mode of Separation SEC / Aqueous Size Exclusion Chromatography (GFC)
Technology HPLC
Matrix Silica
Particle Morphology spherical, fully porous
Number Theoretical Plates ≥ 25,000
Pressure Stability 70 bar

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