PROTEIN KW-804, 8.0 x 300 mm, HPLC column

Part Number: SD-F6989104
Manufacturer: Shodex - Resonac
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Delivery Time: approx. 5 days
Excl. 19% taxes, excl. Shipping costs
Packaging unit: 1 pc, Minimum order quantity: 1 pc
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Product Description

The PROTEIN KW-804 SEC column 300x8.0mm, 7µm, contains spherical silica with a hydrophilic polymer coating with a nominal pore size of 1.500 Å. With a nominal working range of approx. 30.000 to 4.000.000 Da PROTEIN KW-804 primarily addresses large proteins, protein complexes and high-molecular-weight aggregates. The wide-pore matrix extends separation to larger biomolecules. The ranges are nominal guidance values and depend on molecule shape, eluent and calibration.

Features and Benefits

  • Silica-based Bio-SEC phases
  • Defined pores for different protein sizes
  • High efficiency and low adsorption
  • Analytical and semi-micro formats
  • Fast 1.9-µm version available

Additional Notes

The PROTEIN Series uses spherical, porous silica with a hydrophilic polymer coating. The coating reduces hydrophobic and ionic interactions, allowing size-based separation of proteins to take center stage. Different pore sizes target peptides, antibodies, aggregates, and very large protein complexes. Particle sizes and column IDs range from robust routine formats to highly efficient semi-micro and fast SEC columns.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. Protein Series is a product line within the Shodex portfolio.

Product Specification

Flow Rate [ml/min] 1.0
Brand PROTEIN
Product Type SEC/GPC Columns
Length 300 mm
Internal Diameter 8.0 mm
Particle Size 7 µm
Chemistry Producers Term KW
Pore Size 500 Ã…
USP Class L20, L33, L59
Country of Origin Japan
Column Type Analytical Column
Lower Molecular Weight 20.000 (Pullulan), 30.000 (Protein)
Upper Molecular Weight 300.000 (Pullulan), 4.000.000 (Protein)
Exclusion Limit 500.000 (Pullulan), 4.000.000 (Protein)
Solvent H2O
Hardware Material Stainless Steel
Hardware Type Ready-to-use Column
pH Minimum 3.0
pH Maximum 7.5
Temperature Minimum [°C] 4
Temperature Maximum [°C] 45
Mode of Separation SEC / Aqueous Size Exclusion Chromatography (GFC)
Technology HPLC
Matrix Silica
Particle Morphology spherical, fully porous
Number Theoretical Plates ≥ 16,000
Pressure Stability 50 bar

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