PROTEIN KW-802.5, 8.0 x 300 mm, HPLC column

Part Number: SD-F6989000
Manufacturer: Shodex - Resonac
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Delivery Time: approx. 5 days
Excl. 19% taxes, excl. Shipping costs
Packaging unit: 1 pc, Minimum order quantity: 1 pc
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Product Description

The PROTEIN KW-802.5 SEC column 300x8.0mm, 5µm, contains spherical silica with a hydrophilic polymer coating with a nominal pore size of 400 Å. In aqueous size-exclusion chromatography of biomolecules PROTEIN KW-802.5 covers a typical molecular weight range of approx. 5.000 to 100.000 Da and is particularly suitable for peptides, small to medium-sized proteins and protein fragments. The smaller pore provides high differentiation in the lower protein range. The ranges are nominal guidance values and depend on molecule shape, eluent and calibration.

Features and Benefits

  • Silica-based Bio-SEC phases
  • Defined pores for different protein sizes
  • High efficiency and low adsorption
  • Analytical and semi-micro formats
  • Fast 1.9-µm version available

Additional Notes

The PROTEIN Series uses spherical, porous silica with a hydrophilic polymer coating. The coating reduces hydrophobic and ionic interactions, allowing size-based separation of proteins to take center stage. Different pore sizes target peptides, antibodies, aggregates, and very large protein complexes. Particle sizes and column IDs range from robust routine formats to highly efficient semi-micro and fast SEC columns.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. Protein Series is a product line within the Shodex portfolio.

Product Specification

Flow Rate [ml/min] 1.0
Brand PROTEIN
Product Type SEC/GPC Columns
Length 300 mm
Internal Diameter 8.0 mm
Particle Size 5 µm
Chemistry Producers Term KW
Pore Size 150 Ã…
USP Class L20, L33, L59
Country of Origin Japan
Column Type Analytical Column
Lower Molecular Weight 2.000 (Pullulan), 5.000 (Protein)
Upper Molecular Weight 50.000 (Pullulan), 100.000 (Protein)
Exclusion Limit 60.000 (Pullulan), 150.000 (Protein)
Solvent H2O
Hardware Material Stainless Steel
Hardware Type Ready-to-use Column
pH Minimum 3.0
pH Maximum 7.5
Temperature Minimum [°C] 4
Temperature Maximum [°C] 45
Mode of Separation SEC / Aqueous Size Exclusion Chromatography (GFC)
Technology HPLC
Matrix Silica
Particle Morphology spherical, fully porous
Number Theoretical Plates ≥ 21,000
Pressure Stability 50 bar

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