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The Asahipak NH2P-50 HPLC column 250x10mm, 5µm, based on an amino-functionalised polyvinyl alcohol matrix. Asahipak NH2P-50 is used in HILIC and is less susceptible to the hydrolytic ageing processes typical for amino-modified silica phases. In addition to sugar analysis, the robust phase is suitable for ELSD, CAD and LC-MS methods; if performance declines, the polymer material can be regenerated under suitable alkaline conditions.
The Asahipak product family is primarily based on porous polyvinyl alcohol particles. The hydrophilic polymer backbone is chemically stable and, compared to silica, produces only minimal undesirable interactions with basic compounds or biomolecules. Reverse-phase, HILIC, ion exchange, and size exclusion chromatography are supported through C18, C4, amino, and ion-exchange modifications, as well as defined pore sizes. Same-phase guard columns protect the packing bed from particles and highly retained matrix components.
Asahipak is a registered trademark of the manufacturer, Resonac Corporation, Tokyo, Japan.
| Flow Rate [ml/min] | 2.0 to 3.0 |
|---|---|
| Brand | Asahipak |
| Product Type | HPLC Column |
| Length | 250 mm |
| Internal Diameter | 10 mm |
| Particle Size | 5 µm |
| Chemistry Producers Term | NH2P |
| Pore Size | 100 Ã… |
| USP Class | L82 |
| Country of Origin | Japan |
| Column Type | Semi-preparative Column |
| Solvent | CH3CN |
| Base Material | Polyvinylalcohol |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 13.0 |
| Temperature Minimum [°C] | 4 |
| Temperature Maximum [°C] | 50 |
| Mode of Separation | Hydrophilic Interaction Chromatography (HILIC) |
| Technology | HPLC |
| Matrix | Polymer |
| Number Theoretical Plates | ≥ 10,000 |
| Pressure Stability | 150 bar |