Asahipak GS-520 20G 500x20mm Gel Filtration (GFC) Multimode Preparative Column

The Asahipak GS-520 preparative SEC or multimode column 500x20mm, 13µm, contains a porous polyvinyl alcohol gel with a nominal pore size of 2,000 Å. Asahipak GS-520 typically covers a molecular weight range of approximately 5,000 to 200,000 Da for pullulan with an exclusion limit of approximately 300,000 Da. The large-volume format enables higher loadings and the preparative fractionation of proteins, polysaccharides and larger water-soluble polymers. By means of the eluent composition, hydrophobic and ionic contributions can additionally be utilised. The stated molecular weight ranges are nominal, calibration-dependent orientation values.

Features and Benefits

• Hydrophilic polyvinyl alcohol matrix
• Multiple separation mechanisms available
• Broad solvent and pH compatibility
• Low nonspecific adsorption
• Analytical and preparative formats

Additional Information

The Asahipak product family is primarily based on porous polyvinyl alcohol particles. The hydrophilic polymer backbone is chemically stable and, compared to silica, produces only minimal undesirable interactions with basic compounds or biomolecules. Reverse-phase, HILIC, ion exchange, and size exclusion chromatography are supported through C18, C4, amino, and ion-exchange modifications, as well as defined pore sizes. Same-phase guard columns protect the packing bed from particles and highly retained matrix components.

Legal Information

Asahipak is a registered trademark of the manufacturer, Resonac Corporation, Tokyo, Japan.