Asahipak GS-620 20F 300x20mm Gel Filtration (GFC) Multimode Preparative Column

The Asahipak GS-620 preparative SEC or multimode column 300x20mm, 13µm, is packed with a wide-pore polyvinyl alcohol gel with a nominal pore size of 7,000 Å. Asahipak GS-620 typically covers a molecular weight range of approximately 10,000 to 800,000 Da for pullulan with an exclusion limit of approximately 1,000,000 Da. The preparative bed is used for fractionation of high-molecular-weight proteins, polysaccharides, nucleic acids and other water-soluble macromolecules. The multimode selectivity can be influenced by pH, salt concentration and the organic content of the eluent. The stated molecular weight ranges are nominal, calibration-dependent guideline values.

Features and Benefits

• Hydrophilic polyvinyl alcohol matrix
• Multiple separation mechanisms available
• Broad solvent and pH compatibility
• Low nonspecific adsorption
• Analytical and preparative formats

Additional Information

The Asahipak product family is primarily based on porous polyvinyl alcohol particles. The hydrophilic polymer backbone is chemically stable and, compared to silica, produces only minimal undesirable interactions with basic compounds or biomolecules. Reverse-phase, HILIC, ion exchange, and size exclusion chromatography are supported through C18, C4, amino, and ion-exchange modifications, as well as defined pore sizes. Same-phase guard columns protect the packing bed from particles and highly retained matrix components.

Legal Information

Asahipak is a registered trademark of the manufacturer, Resonac Corporation, Tokyo, Japan.