IEC CM-5025 300x50mm Ion Exchange Preparative Column

The Shodex IEC CM-5025 preparative HPLC column „300x50mm, 37µm, is based on a large-pore polyhydroxymethacrylate gel with carboxymethyl groups as a weak cation exchanger. The coarse-particle material enables high loadings and transfers the mild, pH-dependent CM selectivity to large-volume purifications. Shodex IEC CM-5025 is used particularly for proteins, peptides and enzymes where gentle binding and elution conditions are required.

Features and Benefits

• Strong and weak ion exchangers
• Anion and cation phases
• Large-pore materials for biomolecules
• Non-porous option for rapid separations
• Hydrophilic polymer backbones

Additional Notes

The IEX Series combines polyhydroxymethacrylate, polyvinyl alcohol, and styrene-divinylbenzene frameworks. Quaternary ammonium and sulfopropyl groups act as strong ion exchangers, while DEAE and carboxymethyl groups act as weak ion exchangers. Large-pore packing improves access for proteins and nucleic acids, while nonporous particles offer particularly short transport paths. The choice of phase depends on net charge, isoelectric point, molecular size, and the desired elution strategy.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. The IEX Series is a product line within the Shodex portfolio.