OHpak LB-804, 8.0 x 300 mm, HPLC column

The OHpak LB-804 low-bleed SEC column 300x8.0mm, 10µm, contains a controlled low-bleed polyhydroxy methacrylate gel with a nominal pore size of 2,000 Å. In aqueous size-exclusion chromatography, OHpak LB-804 covers a typical molecular weight range of approximately 5,000 to 400,000 Da and is particularly suitable for polysaccharides and larger water-soluble polymers. The reduced release of soluble polymer components improves coupling with light-scattering, viscosity and other sensitive detectors. The stated molecular weight ranges are nominal, calibration-dependent guideline values.

Features and Benefits

• Hydrophilic methacrylate-based SEC gels
• Very wide molecular weight range
• Low-bleed phases for light-scattering detection
• Aqueous and selected polar organic eluents
• Analytical and preparative formats

Additional Notes

OHpak is based on porous polyhydroxymethacrylate gels with graded or mixed pore distributions. The hydrophilic polymer surface is optimized for aqueous SEC and can also be adapted for use with DMF in many SB and LB phases; exceptions and adaptation procedures depend on the specific product. The LB series features controlled, low column bleeding for MALS and viscosity detectors, while the SB-2000 series allows for higher loadings on a preparative scale.

Legal Information

OHpak is a registered trademark of the manufacturer Resonac Corporation, Tokyo, Japan.