IEC DEAE-825, 8.0 x 75 mm, HPLC column

The Shodex IEC DEAE-825 HPLC column 75x8.0mm, 8µm, is based on diethylaminoethyl groups as a weak anion exchanger on a macroporous polyhydroxymethacrylate gel. The charge strength of the functional groups changes with pH and enables finely tuned anion-exchange selectivity. The hydrophilic 5,000Å base matrix reduces hydrophobic secondary interactions. Typical applications of the Shodex IEC DEAE-825 are proteins, enzymes, nucleic acids and sensitive biomolecules.

Features and Benefits

• Strong and weak ion exchangers
• Anion and cation phases
• Large-pore materials for biomolecules
• Non-porous option for rapid separations
• Hydrophilic polymer backbones

Additional Notes

The IEX Series combines polyhydroxymethacrylate, polyvinyl alcohol, and styrene-divinylbenzene frameworks. Quaternary ammonium and sulfopropyl groups act as strong ion exchangers, while DEAE and carboxymethyl groups act as weak ion exchangers. Large-pore packing improves access for proteins and nucleic acids, while nonporous particles offer particularly short transport paths. The choice of phase depends on net charge, isoelectric point, molecular size, and the desired elution strategy.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. The IEX Series is a product line within the Shodex portfolio.