IEC SP-420N 35x4.6mm Ion exchange

The Shodex IEC SP-420N HPLC column 35x4.6mm, 2.5µm, contains sulfopropyl groups as a strong cation exchanger on non-porous polyhydroxymethacrylate particles. The non-porous phase enables fast protein and peptide separations and a rapid analysis of charge variants thanks to the short mass transport paths. Typical applications of Shodex IEC SP-420N are basic proteins, enzymes and other positively charged biomolecules.

Features and Benefits

• Strong and weak ion exchangers
• Anion and cation phases
• Large-pore materials for biomolecules
• Non-porous option for rapid separations
• Hydrophilic polymer backbones

Additional Notes

The IEX Series combines polyhydroxymethacrylate, polyvinyl alcohol, and styrene-divinylbenzene frameworks. Quaternary ammonium and sulfopropyl groups act as strong ion exchangers, while DEAE and carboxymethyl groups act as weak ion exchangers. Large-pore packing improves access for proteins and nucleic acids, while nonporous particles offer particularly short transport paths. The choice of phase depends on net charge, isoelectric point, molecular size, and the desired elution strategy.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. The IEX Series is a product line within the Shodex portfolio.