IEC PIKESS DEAE-2B 50x2.0mm Ion exchange

The Shodex PIKESS DEAE-2B UHPLC column 50x2.0mm, 2.5µm, uses non-porous polyhydroxymethacrylate particles with diethylaminoethyl groups as a weak anion exchanger. The pressure-stable semi-micro format combines short diffusion paths with low sample and eluent consumption and supports very fast charge separations. Shodex PIKESS DEAE-2B is used especially for proteins, peptides and biomolecule variants when high speed and sensitivity are required.

Features and Benefits

• Strong and weak ion exchangers
• Anion and cation phases
• Large-pore materials for biomolecules
• Non-porous option for rapid separations
• Hydrophilic polymer backbones

Additional Notes

The IEX Series combines polyhydroxymethacrylate, polyvinyl alcohol, and styrene-divinylbenzene frameworks. Quaternary ammonium and sulfopropyl groups act as strong ion exchangers, while DEAE and carboxymethyl groups act as weak ion exchangers. Large-pore packing improves access for proteins and nucleic acids, while nonporous particles offer particularly short transport paths. The choice of phase depends on net charge, isoelectric point, molecular size, and the desired elution strategy.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. The IEX Series is a product line within the Shodex portfolio.