IEC QA-825, 8.0 x 75 mm, HPLC column

The Shodex IEC QA-825 HPLC column 75x8.0mm, 12µm contains quaternary ammonium groups as a strong anion exchanger on a macroporous polyhydroxymethacrylate gel. Negatively charged biomolecules are bound via electrostatic interactions and eluted with salt or pH gradients. The 5,000Å pores improve accessibility for large molecules; the exchange capacity is approximately 0.45 meq/g. Typical applications of Shodex IEC QA-825 are proteins, peptides, DNA, RNA and other anionic biopolymers.

Features and Benefits

• Strong and weak ion exchangers
• Anion and cation phases
• Large-pore materials for biomolecules
• Non-porous option for rapid separations
• Hydrophilic polymer backbones

Additional Notes

The IEX Series combines polyhydroxymethacrylate, polyvinyl alcohol, and styrene-divinylbenzene frameworks. Quaternary ammonium and sulfopropyl groups act as strong ion exchangers, while DEAE and carboxymethyl groups act as weak ion exchangers. Large-pore packing improves access for proteins and nucleic acids, while nonporous particles offer particularly short transport paths. The choice of phase depends on net charge, isoelectric point, molecular size, and the desired elution strategy.

Legal Information

Shodex is a registered trademark of Resonac Corporation, Tokyo, Japan. The IEX Series is a product line within the Shodex portfolio.