ACE Excel 5 C4 30x4.6mm

The Avantor ACE Excel C4 HPLC/UHPLC column 30x4.6mm, 5µm, is based on a butyl modification for reversed-phase chromatography. The Avantor ACE Excel C4 offers significantly lower hydrophobic retention than C8 or C18 phases and is therefore suitable for analytes that are overly retained on longer-chain alkyl phases. The shorter alkyl chain can also enable milder elution and shorter analysis times for smaller biomolecules. The Excel format supports high efficiency and fast analyses on UHPLC and HPLC systems. Typical applications are peptides and proteins under 5 kDa, phosphatidylethanol-related biomarkers, hydrophobic small molecules and methods where moderate reversed-phase retention is sufficient.

Features and Benefits

• UHPLC/HPLC hardware for high pressure stability and low system dispersion
• Ultra-inert, base-deactivated, fully porous silica particles
• Wide range of phases for rapid method development
• Excellent peak shape and reproducibility for acidic, basic, and neutral analytes
• Particle sizes for high resolution and fast routine analyses

Additional Information

Avantor ACE Excel combines ACE silica chemistry with a UHPLC-compatible column format. The high-purity, base-deactivated silica matrix minimizes secondary interactions and ensures reproducible retention times. The combination of inert silica, controlled ligand bonding, and pressure-stable hardware allows methods to be implemented in both UHPLC and conventional HPLC systems. Compatible Avantor ACE Excel guard cartridges protect the analytical column from the sample matrix and help maintain the performance of the Excel method over extended runs.

Legal Notices

Avantor ACE is a registered trademark of the manufacturer Avantor, Inc., Radnor, USA.