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The CarboSep CHO 782 HPLC column (300 × 7.8 mm, 7 µm) is a Pb²⁺ ligand-exchange phase with 7 per cent cross-linking on a polymeric backbone. The average degree of cross-linking of the CarboSep CHO 782 lies between that of 6 per cent and 8 per cent materials and is optimised for demanding carbohydrate and biomass samples. Typical applications include sugar alcohols, mono- and disaccharides, as well as carbohydrate profiles in confectionery, syrups, foodstuffs and fermentation samples.
The CarboSep series uses polymer-based packing materials with defined counterions and degrees of cross-linking. Unlike conventional silica phases, retention is not based on hydrophobic surface interactions, but rather on ligand exchange and size- or structure-dependent interactions between the carbohydrates and the polymer resin. This allows sugars, sugar alcohols, and oligosaccharides to be separated in highly aqueous eluents. The polymer backbone used is designed for routine applications in carbohydrate analysis; compatible CarboSep pre-column cartridges help keep matrix components out of the main column.
CarboSep is a registered trademark of the manufacturer Concise Separations, San Jose, USA.
| Brand | CarboSep |
|---|---|
| Product Type | HPLC Column |
| Length | 300 mm |
| Internal Diameter | 7.8 mm |
| Particle Size | 7 µm |
| Chemistry Producers Term | CHO-782 |
| Country of Origin | United States of America (USA) |
| Column Type | Analytical Column |
| Base Material | Styrene-divinylbenzene |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| Temperature Maximum [°C] | 95 |
| Mode of Separation | Ligand Exchange Chromatography |
| Technology | HPLC |
| Matrix | Polymer |
| Counter Ion | Lead (Pb2+) |
| Crosslinkage | 7 % |