Glycomix SAX 250x21.2mm HPLC-Column

The Glycomix SAX HPLC column 250x21.2mm, operates as a strong anion exchanger and is designed for the analysis of heparin sodium. The support consists of narrowly distributed hydrophilic polymer particles onto whose surface quaternary ammonium groups are chemically bound. Because no silica is used, the column can be used from pH 2 to pH 12 and tolerates salt concentrations between 20 mM and 2.0 M. The eluent is a phosphate gradient with sodium perchlorate at pH 3, detected in the shortwave ultraviolet range at 202 nm. Cleaning is performed with potassium nitrate in acetonitrile at acidic pH; the column is stored in water with 20 percent ethanol. As an application example, the testing of heparin sodium for dermatan sulphate and oversulphated chondroitin sulphate is documented, in which both impurities are separated well above the required resolution.

Features and Benefits

• Powerful polymer-based anion exchanger
• Designed for heparin quality control
• Removes dermatan sulfate and OSCS
• pH range: 2 to 12
• Hydrophilic surface without silica
• Suitable for analytical and preparative applications

Additional Notes

Glycomix is not a general-purpose ion-exchange column, but is tailored to a single analytical task: the purity control of heparin sodium. The column therefore consists of exactly one phase. The support is a narrowly distributed hydrophilic polymer particle rather than silica gel, which is why the usable pH range extends from 2 to 12 and even strong cleaning solutions can be used. Chemically bound quaternary ammonium groups are present on this surface, acting as a strong anion exchanger that separates the strongly negatively charged glycosaminoglycans based on charge density. In practice, this means that dermatan sulfate, heparin, and supersulfated chondroitin sulfate elute in that order and with a distinct separation. Oversulfated chondroitin sulfate is the impurity that led to fatalities following heparin administration in 2008 and has since been tested in accordance with pharmacopoeia standards. Regarding the abbreviations: SAX stands for Strong Anion Exchange, DS for dermatan sulfate, and OSCS for oversulfated chondroitin sulfate.

Legal Information

Glycomix is a product of Sepax Technologies, Inc., Newark, Delaware, USA. Sepax uses the names Sepax and Glycomix as trademarks. The product and phase names mentioned are part of the manufacturer’s chromatography portfolio.