Guard Kit Sepax Glycomix SAX, 10x21.2mm

The Sepax Glycomix SAX guard column 10x21.2mm, is used to protect Glycomix SAX HPLC columns. As these are prepacked guard columns, no additional hardware is required.

Features and Benefits

• Powerful polymer-based anion exchanger
• Designed for heparin quality control
• Removes dermatan sulfate and OSCS
• pH range: 2 to 12
• Hydrophilic surface without silica
• Suitable for analytical and preparative applications

Additional Notes

Glycomix is not a general-purpose ion-exchange column, but is tailored to a single analytical task: the purity control of heparin sodium. The column therefore consists of exactly one phase. The support is a narrowly distributed hydrophilic polymer particle rather than silica gel, which is why the usable pH range extends from 2 to 12 and even strong cleaning solutions can be used. Chemically bound quaternary ammonium groups are present on this surface, acting as a strong anion exchanger that separates the strongly negatively charged glycosaminoglycans based on charge density. In practice, this means that dermatan sulfate, heparin, and supersulfated chondroitin sulfate elute in that order and with a distinct separation. Oversulfated chondroitin sulfate is the impurity that led to fatalities following heparin administration in 2008 and has since been tested in accordance with pharmacopoeia standards. Regarding the abbreviations: SAX stands for Strong Anion Exchange, DS for dermatan sulfate, and OSCS for oversulfated chondroitin sulfate.

Legal Information

Glycomix is a product of Sepax Technologies, Inc., Newark, Delaware, USA. Sepax uses the names Sepax and Glycomix as trademarks. The product and phase names mentioned are part of the manufacturer’s chromatography portfolio.