BioBasic SEC300 5µm 50x21.1mm

The BioBasic SEC 300 SEC column 50x2.1mm, 5µm, is based on hydrophilic silica with a nominal pore size of approximately 300 Å. The size separation is tuned for proteins and typical biopharmaceutical size variants. The pore volume enables differentiation of monomers, fragments and aggregates. BioBasic SEC 300 is used particularly for proteins, antibody fragments, enzymes and aggregates.

Features and Benefits

• Wide-pore silica phases for biomolecules
• Reverse-phase, ion exchange, and size exclusion
• Graded hydrophobicity from C18 to C4
• SEC pore sizes for different molecular weights
• Analytical and biocompatible applications

Additional Information

BioBasic includes large-pore silica-based phases for proteins, peptides, oligonucleotides, and other biomolecules. Alkyl, phenyl, and cyano surfaces cover reverse-phase chromatography; AX and SCX cover charge separation; and SEC 60 through SEC 1000 cover various hydrodynamic size ranges. In SEC, pore size and the calibration-dependent fractionation range are critical; in ion exchange, pH and ionic strength determine binding.

Legal Information

Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. BioBasic refers to a product line within the Thermo Scientific chromatography portfolio.