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The BioBasic SEC 120 SEC column 150x8.0mm, 5µm, is made of hydrophilic silica with a nominal pore size of approximately 120 Å. The SEC captures small proteins and peptides via size-dependent pore access. The average pore size expands the working range compared with SEC 60 while maintaining good resolution of small species. BioBasic SEC 120 is used particularly for peptides, protein fragments and small proteins.
BioBasic includes large-pore silica-based phases for proteins, peptides, oligonucleotides, and other biomolecules. Alkyl, phenyl, and cyano surfaces cover reverse-phase chromatography; AX and SCX cover charge separation; and SEC 60 through SEC 1000 cover various hydrodynamic size ranges. In SEC, pore size and the calibration-dependent fractionation range are critical; in ion exchange, pH and ionic strength determine binding.
Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. BioBasic refers to a product line within the Thermo Scientific chromatography portfolio.
| Brand | BioBasic |
|---|---|
| Product Type | SEC / GPC Columns |
| Length | 150 mm |
| Internal Diameter | 8.0 mm |
| Particle Size | 5 µm |
| Chemistry | SEC |
| Chemistry Producers Term | SEC 120 |
| Pore Size | 120 Å |
| USP Class | L59 |
| Carbon Load | 8.2 % |
| Bet Surface Area [m2/g] | 300.0 |
| Endcapping | non endcapped |
| Country of Origin | United States of America (USA) |
| Column Type | Semi-preparative Column |
| Lower Molecular Weight | 300 (Protein), 300 (Pullulan), 400 (PEG/PEO) |
| Upper Molecular Weight | 10.000 (PEG/PEO), 12.000 (Protein), 50.000 (Pullulan) |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Temperature Maximum [°C] | 60 |
| Mode of Separation | SEC / Aqueous Size Exclusion Chromatography (GFC) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |
| Pressure Stability | 295 bar |