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The Thermo Scientific BioBasic SEC 120 Guard Columns 20x8.0mm, 5µm, are used to protect BioBasic HPLC columns with 8.0mm internal diameter installed. As these are pre-packed columns, no additional holder is required.
BioBasic includes large-pore silica-based phases for proteins, peptides, oligonucleotides, and other biomolecules. Alkyl, phenyl, and cyano surfaces cover reverse-phase chromatography; AX and SCX cover charge separation; and SEC 60 through SEC 1000 cover various hydrodynamic size ranges. In SEC, pore size and the calibration-dependent fractionation range are critical; in ion exchange, pH and ionic strength determine binding.
Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. BioBasic refers to a product line within the Thermo Scientific chromatography portfolio.
| Brand | BioBasic |
|---|---|
| Product Type | SEC / GPC Guard Columns |
| Length | 20 mm |
| Internal Diameter | 8.0 mm |
| Particle Size | 5 µm |
| Chemistry | SEC |
| Chemistry Producers Term | SEC 120 |
| Pore Size | 120 Å |
| USP Class | L59 |
| Carbon Load | 8.2 % |
| Bet Surface Area [m2/g] | 300.0 |
| Endcapping | non endcapped |
| Country of Origin | United States of America (USA) |
| Lower Molecular Weight | 300 (Protein), 300 (Pullulan), 400 (PEG/PEO) |
| Upper Molecular Weight | 10.000 (PEG/PEO), 12.000 (Protein), 50.000 (Pullulan) |
| Hardware Material | Stainless Steel |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Temperature Maximum [°C] | 60 |
| Mode of Separation | SEC / Aqueous Size Exclusion Chromatography (GFC) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |
| Pressure Stability | 295 bar |