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The BioBasic SEC 60 SEC column 300x7.8mm, 5µm, based on hydrophilic silica with a nominal pore size of approximately 60 Å. The size-exclusion chromatography is targeted at small biomolecules and peptides. The narrow pores provide enhanced resolution particularly in the low hydrodynamic size range. BioBasic SEC 60 is used especially for peptides, small oligomers and low-molecular-weight biomolecules.
BioBasic includes large-pore silica-based phases for proteins, peptides, oligonucleotides, and other biomolecules. Alkyl, phenyl, and cyano surfaces cover reverse-phase chromatography; AX and SCX cover charge separation; and SEC 60 through SEC 1000 cover various hydrodynamic size ranges. In SEC, pore size and the calibration-dependent fractionation range are critical; in ion exchange, pH and ionic strength determine binding.
Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. BioBasic refers to a product line within the Thermo Scientific chromatography portfolio.
| Brand | BioBasic |
|---|---|
| Product Type | SEC / GPC Columns |
| Length | 300 mm |
| Internal Diameter | 7.8 mm |
| Particle Size | 5 µm |
| Chemistry | Diol |
| Chemistry Producers Term | SEC 60 |
| Pore Size | 60 Å |
| USP Class | L20 |
| Carbon Load | 9.0 % |
| Bet Surface Area [m2/g] | 450.0 |
| Endcapping | non endcapped |
| Country of Origin | United States of America (USA) |
| Column Type | Semi-preparative Column |
| Lower Molecular Weight | 100 (PEG/PEO), 100 (Protein), 300 (Pullulan) |
| Upper Molecular Weight | 4.000 (PEG/PEO), 6.000 (Protein), 6.000 (Pullulan) |
| Hardware Material | Stainless Steel |
| Hardware Type | Ready-to-use Column |
| pH Minimum | 2.0 |
| pH Maximum | 8.0 |
| Temperature Maximum [°C] | 60 |
| Mode of Separation | SEC / Aqueous Size Exclusion Chromatography (GFC) |
| Technology | HPLC |
| Matrix | Silica |
| Particle Morphology | spherical, fully porous |
| Pressure Stability | 295 bar |