BioBasic SEC 60 5µm 300x78mm Prep Column

Part Number: TFS-73305-307846A
Manufacturer: Thermo Scientific
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Delivery Time: approx. 6 days
Excl. 19% taxes, excl. Shipping costs
Packaging unit: 1 pc, Minimum order quantity: 1 pc
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Product Description

The BioBasic SEC 60 SEC column 300x7.8mm, 5µm, based on hydrophilic silica with a nominal pore size of approximately 60 Å. The size-exclusion chromatography is targeted at small biomolecules and peptides. The narrow pores provide enhanced resolution particularly in the low hydrodynamic size range. BioBasic SEC 60 is used especially for peptides, small oligomers and low-molecular-weight biomolecules.

Features and Benefits

  • Wide-pore silica phases for biomolecules
  • Reverse-phase, ion exchange, and size exclusion
  • Graded hydrophobicity from C18 to C4
  • SEC pore sizes for different molecular weights
  • Analytical and biocompatible applications

Additional Information

BioBasic includes large-pore silica-based phases for proteins, peptides, oligonucleotides, and other biomolecules. Alkyl, phenyl, and cyano surfaces cover reverse-phase chromatography; AX and SCX cover charge separation; and SEC 60 through SEC 1000 cover various hydrodynamic size ranges. In SEC, pore size and the calibration-dependent fractionation range are critical; in ion exchange, pH and ionic strength determine binding.

Legal Information

Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. BioBasic refers to a product line within the Thermo Scientific chromatography portfolio.

Product Specification

Brand BioBasic
Product Type SEC / GPC Columns
Length 300 mm
Internal Diameter 7.8 mm
Particle Size 5 µm
Chemistry Diol
Chemistry Producers Term SEC 60
Pore Size 60 Å
USP Class L20
Carbon Load 9.0 %
Bet Surface Area [m2/g] 450.0
Endcapping non endcapped
Country of Origin United States of America (USA)
Column Type Semi-preparative Column
Lower Molecular Weight 100 (PEG/PEO), 100 (Protein), 300 (Pullulan)
Upper Molecular Weight 4.000 (PEG/PEO), 6.000 (Protein), 6.000 (Pullulan)
Hardware Material Stainless Steel
Hardware Type Ready-to-use Column
pH Minimum 2.0
pH Maximum 8.0
Temperature Maximum [°C] 60
Mode of Separation SEC / Aqueous Size Exclusion Chromatography (GFC)
Technology HPLC
Matrix Silica
Particle Morphology spherical, fully porous
Pressure Stability 295 bar

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