BioBasic SCX 5µm 100x4.6mm Column

The BioBasic SCX HPLC column 100x4.6mm, 5µm, consists of macroporous silica with strong cation-exchange groups. The negatively charged surface binds cationic biomolecules depending on pH and ionic strength. The phase provides high charge selectivity with good accessibility for proteins and peptides. BioBasic SCX is used particularly for protein variants, peptides, basic proteins and biotherapeutics.

Features and Benefits

• Wide-pore silica phases for biomolecules
• Reverse-phase, ion exchange, and size exclusion
• Graded hydrophobicity from C18 to C4
• SEC pore sizes for different molecular weights
• Analytical and biocompatible applications

Additional Information

BioBasic includes large-pore silica-based phases for proteins, peptides, oligonucleotides, and other biomolecules. Alkyl, phenyl, and cyano surfaces cover reverse-phase chromatography; AX and SCX cover charge separation; and SEC 60 through SEC 1000 cover various hydrodynamic size ranges. In SEC, pore size and the calibration-dependent fractionation range are critical; in ion exchange, pH and ionic strength determine binding.

Legal Information

Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. BioBasic refers to a product line within the Thermo Scientific chromatography portfolio.