BioBasic AX 5µm 250x4.6mm Column

The BioBasic AX HPLC column 250x4.6mm, 5µm, is used with porous silica featuring strong anion-exchange groups. The positively charged surface binds anionic biomolecules and elutes them using salt or pH gradients. The large pore access supports charge separations of macromolecules. BioBasic AX is used particularly for oligonucleotides, nucleic acids, acidic proteins and phosphorylated biomolecules.

Features and Benefits

• Wide-pore silica phases for biomolecules
• Reverse-phase, ion exchange, and size exclusion
• Graded hydrophobicity from C18 to C4
• SEC pore sizes for different molecular weights
• Analytical and biocompatible applications

Additional Information

BioBasic includes large-pore silica-based phases for proteins, peptides, oligonucleotides, and other biomolecules. Alkyl, phenyl, and cyano surfaces cover reverse-phase chromatography; AX and SCX cover charge separation; and SEC 60 through SEC 1000 cover various hydrodynamic size ranges. In SEC, pore size and the calibration-dependent fractionation range are critical; in ion exchange, pH and ionic strength determine binding.

Legal Information

Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. BioBasic refers to a product line within the Thermo Scientific chromatography portfolio.