BioBasic 8 5µm 250x4.6mm Column

The BioBasic 8 HPLC column 250x4.6mm, 5µm, is based on wide-pore 300Å silica with a C8 phase (other designations: RP-8, C8). The shorter alkyl chain produces lower hydrophobic retention than BioBasic 18. This results in elution of large or strongly hydrophobic biomolecules with milder gradients and often higher recovery. BioBasic 8 is used particularly for peptides, proteins and hydrophobic biomolecules.

Features and Benefits

• Wide-pore silica phases for biomolecules
• Reverse-phase, ion exchange, and size exclusion
• Graded hydrophobicity from C18 to C4
• SEC pore sizes for different molecular weights
• Analytical and biocompatible applications

Additional Information

BioBasic includes large-pore silica-based phases for proteins, peptides, oligonucleotides, and other biomolecules. Alkyl, phenyl, and cyano surfaces cover reverse-phase chromatography; AX and SCX cover charge separation; and SEC 60 through SEC 1000 cover various hydrodynamic size ranges. In SEC, pore size and the calibration-dependent fractionation range are critical; in ion exchange, pH and ionic strength determine binding.

Legal Information

Thermo Scientific is a brand name of Thermo Fisher Scientific Inc. BioBasic refers to a product line within the Thermo Scientific chromatography portfolio.