Ascentis® RP-Amide HPLC Column, 5 µm particle size, L x I.D. 25 cm x 4.6 mm

The Ascentis RP-Amide HPLC column 250x4.6mm, 5µm, uses an amide-embedded alkyl phase for reversed-phase chromatography. Due to the polar amide group, the Ascentis RP-Amide exhibits additional hydrogen-bonding and dipole interactions, enabling better retention of polar analytes in RP methods. Typical fields of application are active pharmaceutical ingredient analysis, pharmaceutical impurities, metabolites and samples where a standard C18 phase is not sufficiently selective.

Features and Benefits

• High-purity Type B silica
• Wide selection of RP, NP, and specialty phases
• Good peak shapes for acidic, neutral, and basic analytes
• Analytical and preparative formats available
• Compatible Supelguard column protection systems

Additional Notes

The Ascentis series is based on spherical, high-purity silica with a controlled pore structure. Depending on the modification, hydrophobic, polar, aromatic, or normal-phase interactions are emphasized. As a result, Ascentis can be used for both classical reverse-phase methods and more selective method developments. The use of pre-column cartridges or guard columns in the same phase protects the analytical column from particles and highly retained matrix components without significantly altering the selectivity of the method.

Legal Information

Ascentis is a registered trademark of the manufacturer Merck KGaA, Darmstadt, Germany.