Inertsil WP300 C18 3.5µm, HPLC-Column 100x3.0mm HPLC Column

The Inertsil WP300-C18 HPLC column 100x3.0mm, 3.5µm, is based on an octadecyl modification (also known as ODS, RP-18, C18) on wide-pore silica with a pore width of 300 Å. The larger pores facilitate the transport of large molecules and enable reproducible reverse-phase separations of biomolecules. Inertsil WP300 C18 is particularly suitable for proteins, peptides, and larger hydrophobic molecules, for which standard pores may restrict access to the internal surface. Typical applications include peptide and protein analysis, biopharmaceuticals, peptide mapping, purity testing, and bioanalytical methods.

Performance Characteristics and Benefits

• High-purity silica
• Controlled pore structure and reproducible surface
• High inertness due to reduced silanol and metal activity
• Excellent peak shapes for acidic, neutral, and basic analytes
• Reliable reproducibility for routine analysis

Additional Notes

The Inertsil series is based on a high-purity, spherical silica matrix whose pore structure, particle size distribution, and surface are carefully controlled during production. Low metal contamination and careful surface treatment reduce the activity of free silanol groups. This minimizes undesirable secondary interactions—particularly tailing and adsorption with basic or chelating analytes—and promotes stable retention times. This reproducible silica matrix forms the basis for the various Inertsil modifications and ensures reliable selectivities across the series. Appropriate Inertsil pre-column cartridges should be used to protect the analytical packing bed from particles and the sample matrix and to extend column lifespan.

Legal Notices

Inertsil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.