PROTEOSIL 300-C18 10µm, HPLC-Column 250x4.6mm HPLC Column

The ProteoSil 300-C18 HPLC column 250x4.6mm, 10µm, is based on a C18 modification on wide-pore 300 Å silica. With this pore size, the ProteoSil 300-C18 is designed for larger proteins, hydrophobic peptides, oligonucleotides, and nucleic acids. The C18 modification provides strong hydrophobic retention and can be used when C4 or C8 phases provide insufficient retention or sample capacity. Typical applications include protein analysis, biopharmaceutical methods, LC/MS analysis of larger biomolecules, and separations requiring high retention of large hydrophobic structures.

Features and Benefits

• Bio-LC column series for proteins, peptides, oligonucleotides, nucleic acids, and biopharmaceutical applications
• High-purity silica with controlled pore sizes of 100 Å, 200 Å, and 300 Å
• Choice between reverse-phase, HILIC, and size-exclusion chromatography
• Stainless steel, PEEK, and bio-inert hardware for various requirements
• Low lot-to-lot variability and consistent quality, even for LC/MS methods

Additional Information

The ProteoSil series is designed for bioseparations in which pore size, modification, and hardware must be specifically tailored to sensitive biomolecules. It is based on a high-purity silica matrix with pore sizes of 100 Å, 200 Å, or 300 Å. Smaller pores are particularly suitable for low-molecular-weight peptides, while 200 Å materials are used for medium-sized peptides and oligonucleotides. For larger proteins, hydrophobic peptides, and antibodies, 300 Å materials are available. In addition, ProteoSil HILIC and ProteoSil SEC round out the series for hydrophilic analytes and size-based separations. Using appropriate ProteoSil pre-column cartridges or guard columns, the analytical column can be protected from particles, strongly adsorbing sample components, and matrix carryover.

Legal Information

ProteoSil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.