ProteoSil 200-C18, UHPLC PEEK Analytical Column 3um, 2.1x100mm HPLC Column

The ProteoSil 200-C18 HPLC column 100x2.1mm, 3µm, features a C18 modification on high-purity silica with a pore size of 200 Å. Due to its larger pores compared to 100 Å materials, the ProteoSil 200-C18 is particularly suitable for medium-sized biomolecules. Typical analytes include peptides, oligonucleotides, smaller proteins, and nucleic acids ranging from a few kDa to several tens of kDa. The C18 surface provides pronounced hydrophobic retention, while the 200 Å pores facilitate the access of larger molecules to the stationary phase. This phase is primarily used for peptide mapping, oligonucleotide analysis, and LC/MS methods involving molecules of medium size.

Features and Benefits

• Bio-LC column series for proteins, peptides, oligonucleotides, nucleic acids, and biopharmaceutical applications
• High-purity silica with controlled pore sizes of 100 Å, 200 Å, and 300 Å
• Choice between reverse-phase, HILIC, and size-exclusion chromatography
• Stainless steel, PEEK, and bio-inert hardware for various requirements
• Low lot-to-lot variability and consistent quality, even for LC/MS methods

Additional Information

The ProteoSil series is designed for bioseparations in which pore size, modification, and hardware must be specifically tailored to sensitive biomolecules. It is based on a high-purity silica matrix with pore sizes of 100 Å, 200 Å, or 300 Å. Smaller pores are particularly suitable for low-molecular-weight peptides, while 200 Å materials are used for medium-sized peptides and oligonucleotides. For larger proteins, hydrophobic peptides, and antibodies, 300 Å materials are available. In addition, ProteoSil HILIC and ProteoSil SEC round out the series for hydrophilic analytes and size-based separations. Using appropriate ProteoSil pre-column cartridges or guard columns, the analytical column can be protected from particles, strongly adsorbing sample components, and matrix carryover.

Legal Information

ProteoSil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.