ProteoSil 100-C18 Analytical Column 3um HP, 4.6x75mm HPLC Column

The ProteoSil 100-C18 HPLC column 75x4.6mm, 3µm, is based on an octadecylsilyl modification (other designations: ODS, RP-18, C18) on high-purity silica with a pore size of 100 Å. The C18 phase is used in reverse-phase chromatography and is designed for smaller proteins and peptides, as well as oligonucleotides and nucleic acids with a molecular weight below approximately 5,000 Da. Thanks to its 100 Å pores, ProteoSil 100-C18 provides a large surface area and good retention for low-molecular-weight peptides. Typical applications include peptide analysis, LC/MS methods, protein digests, and bioanalytics, where classic C18 selectivity combined with high inertness and robust peak shape is required.

Features and Benefits

• Bio-LC column series for proteins, peptides, oligonucleotides, nucleic acids, and biopharmaceutical applications
• High-purity silica with controlled pore sizes of 100 Å, 200 Å, and 300 Å
• Choice between reverse-phase, HILIC, and size-exclusion chromatography
• Stainless steel, PEEK, and bio-inert hardware for various requirements
• Low lot-to-lot variability and consistent quality, even for LC/MS methods

Additional Information

The ProteoSil series is designed for bioseparations in which pore size, modification, and hardware must be specifically tailored to sensitive biomolecules. It is based on a high-purity silica matrix with pore sizes of 100 Å, 200 Å, or 300 Å. Smaller pores are particularly suitable for low-molecular-weight peptides, while 200 Å materials are used for medium-sized peptides and oligonucleotides. For larger proteins, hydrophobic peptides, and antibodies, 300 Å materials are available. In addition, ProteoSil HILIC and ProteoSil SEC round out the series for hydrophilic analytes and size-based separations. Using appropriate ProteoSil pre-column cartridges or guard columns, the analytical column can be protected from particles, strongly adsorbing sample components, and matrix carryover.

Legal Information

ProteoSil is a registered trademark of the manufacturer GL Sciences Inc., Tokyo, Japan.